马蓝种质资源的RAPD分析

RAPD Analysis on the Germplasm Resources of Baphicacanthus cusia

目的:探讨马蓝种质资源在分子水平上的遗传多样性。方法:利用随机扩增多态性DNA技术(RAPD)对收集到的不同居群马蓝资源进行分析。结果:从104条10个碱基的随机引物筛选出有差异的有效引物,筛选出的12条有效引物,扩增共获得422条DNA带,多态性程度为55.69%。聚类分析表明,马蓝种质资源的遗传差异与地理位置无密切联系,而与形态特征有一定联系,但并不十分紧密。结论:不同自然居群的马蓝资源在分子水平上存在着较明显的遗传差异。

Objective：To explore the genetic diversity of germplasm resources of Baphicacanthus cusia on molecular leve. Methods： The molecular biological technique-random amplified polymorphic DNA（RAPD）were used . Results： 104 random decamer primers were screened for RAPD fragments of Baphicacanthus cusia.422 DNA bands were amplified by 12 primers,55.69% products were found to be polymorphic.Base on UPGMA cluster analysis,a DNA molecular dendrogram was established to discuss the genetic diversity of the germplasm resources of Baphicacanthus cusia. The genetic differences are related to morphological differences on a certain extant,but not to geographic regions. Conclusion：There actually existed much genetic diversity on molecular level among different natural populations of Baphicacanthus cusia.