摘要
目的:探讨黑水缬草提取物对老年痴呆模型大鼠大脑皮质神经元和海马神经元中β-APP、Aβ1-40和Caspase-3表达的影响。方法:利用多因素造成大鼠老年痴呆病理复合模型,采用免疫组化法观察老年痴呆大鼠大脑皮质神经元和海马神经元中β-APP、Aβ1-40和Caspase-3蛋白表达变化。结果:黑水缬草各给药组和中药对照组显示出一定的疗效(P<0.05),其中以50%乙醇树脂洗脱高剂量效果尤为显著(P<0.05或P<0.01)。结论:黑水缬草50%乙醇树脂洗脱物能有效抑制神经元细胞内β-APP和Aβ1-40阳性细胞过表达,阻止其聚集脑内形成老年斑和神经纤维缠结,并能有效抑制Caspase-3的活化而减少神经元细胞的凋亡。
Objective：To investigate the effects of Valeriana amurensis on the expressions of β-APP、Aβ1-40 and Caspase-3 of cortical neurons and hippocampus neuronsin in Alzheimer′s disease model rats′ brain. Methods：Established the model with multiple factors, observed the expressions of β-APP、Aβ1-40 and Caspase-3 of cortical neurons and Hippocampus neuronsin in Alzheimer′s disease model rats′ brain by electron lens using immunohistochemistry method. Results：The extracts of the roots and rhizomes of Valeriana amurensis decreased the expressions of β-APP,Aβ1-40 and Caspase-3 of cortical neurons and hippocampus neuronsin in a dose-dependent manner. The effects of high dose of 50% ethanol extract of macroporous resin group were more significant than the other groups′. Conclusion：The 50% ethanol extracts of macroporous resin group from the roots and rhizomes of Valeriana amurensis can decrease the expressions of β-APP,Aβ1-40 and Caspase-3,to inhibit the formation of senile plaques and neurofibrillary tangles in Alzheimer′s disease model rats′ brain,and decrease cell fade of cortical neurons and hippocampus neuronsin in Alzheimer′s disease model rats′ brain.
出处
《中药材》
CAS
CSCD
北大核心
2010年第2期233-236,共4页
Journal of Chinese Medicinal Materials
