人成骨样细胞OS-732酒精干预和六味地黄丸含药血清的治疗作用

Human Osteoblast-like Cells OS-732 Intervened with Alcohol and Treated with Liuweidihuang Pill Medicated Serum

目的:阐明酒精性股骨头坏死及补肾中药对其治疗作用的机制。方法:以浓度0.3、0.9、1.5mol/L的酒精对OS-732成骨样细胞进行干预,通过流式细胞仪等观察其坏死和凋亡的情况;选择浓度为0.15mol/L酒精干预后的细胞模型用六味地黄丸含药血清治疗,观察其治疗作用。结果:成骨样细胞凋亡率和坏死的程度与干预酒精的浓度及干预时间正相关,其中多形状的细胞变成圆形,细胞突起短缩变细,核糖体、内质网减少,线粒体部分皱褶消失。经六味地黄丸治疗后的细胞突起恢复,内质网、线粒体数量有所增加,结构部分恢复。结论:酒精可引起成骨样细胞死亡和凋亡,且与酒精浓度及干预时间成正相关,六味地黄丸对恢复细胞的活性有一定作用。

Objective： TO investigate the mechanism of osteonecrosis of the femoral head caused by alconhol and the therapeutic effects of the method of invigorating the kidney. Methdos ： The osteoblast-like ceils were intervened with 0. 3, 0. 9, 1.5 mol/L alcohol and observed by flow cytometry and light microscopy and electro microscopy. The cells intervened by 0. 15 mol/L alcohol were treated with Liuweidihuang Pill medicated serum and observed by Flow Cytometry and light microscopy and electro microscopy. Results： Apoptosis rate and the degree of cell necrosis had postive correlation with the concentration and the acting time of alcohol. The polymorphism of cells wer turned into roundness, and the cellular ecphyma was shortened and thinningz, ribosome and endocytoplasmic reticulum were diminished. Part of the ruffle in mitochondria was disappeared. After treatment, the cellular ecphyma was recovered and the endoplasmic reticulum and golgis apparatus were visible. Conclusion： The osteoblast-like cells could be necrosis and apoptosis by the alco- hol and were positive correlation with the intervened concentration and acting time. Chinese medicine with method of invigorating the kidney has effect on rehabilitating the cellular activity.