HPLC指纹图谱在白花丹提取工艺对比中的应用

Process Comparison of Fingerprints of Different Extracts of Plumbago zeylanica by High Performance Liquid Chromatography

目的:建立并优化白花丹的最佳色谱条件,采用HPLC指纹图谱选择白花丹药材最佳的提取方法。方法:以保留时间、相对峰面积为指标,综合评判,对白花丹半仿生提取法、醇提取法与水提取法进行工艺对比。结果:建立了白花丹最优的HPLC-FPs分离条件,3种提取工艺得到4个共有峰,白花丹醇提物与半仿生提取物的指纹图谱相关性较好,得到9个共有峰。白花丹药材用半仿生提取优于水提和醇提。结论:白花丹半仿生提取有望取代水提、醇提法,为其入血成分、制剂工艺及药效学研究奠定了基础。

Objective：To optimize the separation conditions of fingerprints of Plumbago zeylanica by high performance liquid chromatography, on this basis, to choose the best way for extracting Plumbago zeylanica by high performance liquid chromatography. Methods： The investigation of HPLC-FPs of the different extraction samples was by the retention time and the relative area of common peaks in fingerprints. Results： We established the optimum separation conditions of HPLC-FPs for Plumbago zeylanica and there were 4 common peaks in fingerprints. According to the contrast research, the HPLC-FPs of the 90% ethanol extraction and SBE extraction of Plumbago zeylanica were quite similar, and they both had 9 common seaks in fingerprints, It was better to use SBE rather than WE in the extraction of Plumbago zeylanica. Conclusion： The SBE may replace the WE and ethanol extraction of Plumbago zeylanica.