委陵菜抗糖尿病有效成分提取研究

Study on Extraction of Anti-diabetes Active Constituents from Potentilla chinesis

目的:对委陵菜中一个具有抗糖尿病活性的黄酮类化合物的提取工艺进行系统考察。方法:通过单因素考察,以委陵菜黄酮得率为指标,分别考察了乙醇浓度、提取时间、提取次数和液料比对提取率的影响。并选取提取次数、液料比、乙醇浓度3个因素,设计正交实验。结果:影响委陵菜黄酮提取率的主次因素为提取次数>料液比>提取时间,最佳提取工艺为液料比8-6-6回流提取3次,每次1.5h。结论:优选出了最佳提取工艺条件,为开发中药委陵菜中抗糖尿病活性成分提供了一个科学、合理的提取方法。

Objective：To study the extraction technology of anti-diabetes active constituents from Potentilla chinesis. Methods： Through single factor test, the effects of factors such as concentration of ethanol, temperature, ratio of liquid-solid, extraction time and times of extraction were investigated. Through orthogonal experiment, the optimal conditions of extraction were obtained on the basis of single factor test. Results： The optimum extraction conditions of Potentilla flavone from P.chinesis were determined as follows： refluxing for 3 times,1.5 hours each time, and 8-6-6 for the multiple of 60% EtOH and material. Conclusion： The extraction process is stable and suitable for industry.