期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

雷帕霉素对绒癌JEG-3细胞株的影响 被引量：2

The effection of rapamycin to chorioepithelioma JEG-3 cell line
下载PDF
导出
摘要 用雷帕霉素对人绒癌层细胞株JEG-3进行处理,探讨其对JEG-3的作用。分别采用MTT法、流式细胞术、Transwell技术检测雷帕霉素处理前后JEG-3细胞在增殖、凋亡和侵袭能力的改变。结果显示雷帕霉素对绒癌细胞的增殖、侵袭能力起着抑制作用,并对凋亡起促进作用。 The study discusses the effection of rapamycin in JEG - 3 cell line. Before and after rapa treatment , we test the difference in proliferation, apoptesis and invasion ability of JEG - 3 by MTT techology, FCM teclmology and Tanswell invasion. The results show that rapamycin inhibits proliferation and invasion ability of JEG - 3 and stimulates apoptosis of JEG - 3.
作者 黄玲 陈雪梅 何俊琳 王应雄 丁裕斌 鲁之中 李荣 刘学庆
机构地区 重庆医科大学公共卫生学院生殖生物学研究室
出处 《激光杂志》 CAS CSCD 北大核心 2010年第2期93-94,共2页 Laser Journal
基金 重庆市科委自然科学基金(CSTC 2008BB5232) 重庆市教委自然科学基金(KJ080318)资助项目
关键词 雷帕霉素 绒癌JEG-3 增殖 凋亡 侵袭能力 rapamvein chorioepithelioma JEG - 3 proliferation apoptosis invasion ability
分类号 R477 [医药卫生—护理学]
  • 相关文献

参考文献4

  • 1Rowinsky E K. Targeting the molecular target of rapamycin (mTOR) . Curr Opin Oncol, 2004,16(6) :564 - 575.
  • 2Gera JF, Mellinghoff IK,Shi Y, Rettig MB,Tran C, Hsu JH, Sawers CL, Lichtenstein AK. AKT activity determines sensitivity to mammalian target of rapamycin (mTOR) inhibitors by regulating cyclin DI and c - myc expression. J Biol Chem,2004,23,279(4) :2737 - 2746.
  • 3Fingar DC, Blenis J.Target of rapamycin(TOR) : Target of rapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell cycle progression. Oncogene, 2004 Apr, 19,23 (18):3151-3171.
  • 4Fingar DC, Richardson C J, Tee AR, Cheatham L, Tsou C, Blenis J. rnTOR controls cell cycle progression through its cell growth effectors S6KI and 4E - BP1/eukaryotic translation initiation factor 4E. Mol Cell Biol, 2004 Jan, 24 ( 1 ) : 200 - 216.

同被引文献18

  • 1Rohde J, Heitman J, Cartlenas ME. The TOR kinases link nutrient sensing to cell growth[J] .J Biol Chem, 2001, 279(13):9583- 9586.
  • 2Drenan RM,Liu XY, Bertram PC, et al. FKBP12 - apamycinassociated protein or mannalian Target of rapamycin(FRAP/mTOR) localization in the endoplasmic reticulum and the golgi apparatus [ J]. Biol Chem, 2004,279( 1 ) :772 - 778.
  • 3Fingar DC, Blenis J. Target ofrapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell cycle progression[J].Oncogene,2004, 23( 18 ) : 3151.
  • 4Dilling M B, Germain G S, Dudkin Let al. 4Ebinding proteins, the suppressors of eukaryotic initiation factor 4E, are down - regulated in cells with acquired or intrinsic resistance torapamyein[J] .J Biol Chem, 2002, 277(16):13907- 13917.
  • 5Jefferies H'B j, Fumagalli S,DennisP B,et al. Rapmnyein suppresses 5 TOP mRNA translation inhibition of p70 S6K[J]. EMBO J, 1997, 16: 3693 - 3704.
  • 6Isotani S, Hara K, Yonezawa K. Immunopurified mammalian target of rapamyein phosphorylates and activates p70 S6 kinase a in vltro[J].J Biol Chem, 1999,274:34493- 34498.
  • 7Yoshizawa A,Fukuoka J,Shimizu S,et al.Overexpression of phosphor-eIF4E is associated with survival through AKT pathway in non-small cell lung cancer[J].Clin Cancer Res,2010,16(1):240-248.
  • 8Wang X,Yue P,Kiml YA,et al.Enhancing mTOR-targeted cancer therapy by preventing mTOR/raptor inhibition-initiated,mTOR/rector-independent Akt activation[J].Cancer Res,2008,68(18):7409-7418.
  • 9Guertin DA,Sabatini DM.An expanding role for mTOR in cancer[J].Tremls Mol Med,2005,11(18):353-361.
  • 10Hon JC,Kahan BD.Rapamycin and transplantation[J].Transplantation,1999,68(5):701-704.

引证文献2

二级引证文献2

激光杂志
2010年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部