摘要
本文试验了4种抗生素对柑桔胚性愈伤组织、原生质体生长的影响，认为在柑桔的遗传转化中宜选用潮霉素磷酸转移酶（Hpt）基因为选择标记基因，以潮霉素作为筛选剂，即使是使用新霉素磷酸转移酶（NptⅡ）基因作为选择标记基因，也不宜使用卡那霉素作为筛选剂，同时确定了柑桔遗传转化后的筛选时宜采用的抗生素浓度。
The effects of Kanamycin, Hygromycin B,G418 and Cefotaxime on the division and growth of Citrus embryogenic calli and protoplasts were investigated. When calli and protoplasts were culture with 50μg/ml Kanamycin, 25μg/ml Hygromycin B and 20μg/ml G418 respectively, the division and growth were completely restrained. Cefotaxime had no effect on the diivision of protoplasts. In Citrus transformation, 50μg/ml Hygromycin B and 40μfor selecting resistant cell clones which were been transferred with Hpt gene ahd Npt Ⅱ gene respectively.
出处
《福建果树》
1998年第4期6-9,共4页
Fujian Fruits
基金
福建省自然科学基金
