比较不同温度对乳腺癌侵袭转移能力的影响

Comparison of hyperthermia effects with different temperatures on human breast adenocarcinoma invasiveness and metastasis

目的比较研究不同温度的热疗对人乳腺癌株MCF-7细胞侵袭转移能力的影响及其相关分子机制。方法水浴热疗43、45和47℃3组MCF-7细胞各30min，37℃为对照组。采用免疫组织化学染色法检测 MCF-7细胞转化生长因子-β1（TGF—β1）、血管内皮生长因子（VEGF）蛋白的表达；明胶酶谱法检测细胞上清液中基质金属蛋白酶（MMP）-2、MMP-9的分泌及活性。结果热疗对MMP-2、MMP-9的释放和激活均有明显的抑制作用（P〈0．05）；且对MMP-2活性的抑制作用有明显的温度依赖性，随加热温度增加，抑制作用越明显（P〈0．05）。各热疗组TGF—β1和VEGF的表达均较对照组显著下降（P〈0．05），但不同温度热疗组之间差异无统计学意义（P〉0．05）；结论43、45、47℃热疗均可下调MCF-7细胞中TGF—β1、VEGF的蛋白表达及抑制MMP-2、MMP-9的分泌和活性，从而抑制人乳腺癌细胞侵袭转移能力。

Objective To study the effects of hyperthermia with different temperature on inhibiting invasiveness and metastasis of human breast adenocarcinoma cells（MCF-7） and its possible mechanism. Methods MCF-7 was treated by heating with different temperatures（43 ℃,45 ℃ and 47 ℃ ）for 30 minites and 37 ℃ as control. Expression of matrix metalloproteinase （MMP） - 2, MMP- 9, vascular endothelial growth factor （ VEGF）, transforming growthfactor（TGF）- β1 of MCF-7 after different heating temperatures treatment was examined by immuno-cytochemical technique;The effect of hyperthermia on MMP-2, MMP-9 expression, activity were also studied by gelatin zymograpbic assays. Results Comparing with 37 ℃ group, byperthermia with 43 ℃ ,45 ℃ and 47℃ also decreased MMP-2,MMP-9 enzymatic activifiesl which is temperature-dependant（ P 〈 0.05）. The VEGF,TGF-131 protein expression in 43 ℃ ,45 ℃ and 47 ℃ groups decreased significantly（ P 〈0. 05）, but there is no differeme between heating groups （ P 〉 0. 05）. Conclusion Hyperthermia as potential treat- ment inhibits invasion by a process involved in muhiple regulatory molecules in MCF-7.