氯化甲基汞对人SHG44胶质瘤细胞抗肿瘤活性的体外实验研究

Inhibitory effect of methylmercury chloride on human SHG44 glioma cells in vitro

目的探讨氯化甲基汞(MMC)对人SHG44胶质瘤细胞株的增殖、杀伤和细胞凋亡的影响,为胶质瘤治疗提供理论依据。方法体外培养SHG44胶质瘤细胞,分为空白对照组和MMC实验组(按氯化甲基汞浓度分组)。采用MTT比色法检测不同浓度MMC对体外培养SHG44细胞的增殖抑制和杀伤作用。用流式细胞仪测定MMC对SHG44细胞凋亡/坏死的影响。结果在体外1.25、2.50、5.00和10.00μmol.L-1的MMC均可抑制SHG44胶质瘤细胞的增殖,SHG44胶质瘤细胞存活率明显低于对照组(P<0.05),增殖抑制作用随浓度的增加而增强。SHG44胶质瘤细胞接触2.50、5.00和10.00μmol.L-1的MMC后4、8、16和32 h的细胞存活率明显低于对照组(P<0.05),细胞杀伤作用随浓度的增加和时间的延长而增强。SHG44胶质瘤细胞接触0.075、0.15、0.3和1.2μmol.L-1氯化甲基汞12 h后细胞凋亡/坏死率呈现剂量依赖性增高趋势(P<0.01)。结论MMC具有杀伤SHG44胶质瘤细胞、抑制其增殖、诱导凋亡的抗肿瘤活性,有应用于胶质瘤治疗的潜在价值。

Objective To study the inhibitory effect of methyhnereury chloride（MMC） on human SHG44 glioma cells in vitro. Methods The human SHG44 gallmo cells were cultivated in vitro and divided into control group and MMC- treated group. MMT assay was performed to evaluate the proliferation inhibitory effect and cytotoxicity effect of MMC with different concentrations on cultured human SHG44 glioma cells, and flow cytometry was used to assess the effects of MMC treatment on cell apoptosis in human SGH44 glioma cells. Results 1.25,2.50,5.00 and 10.00 μmol·L^-1 MMC could inhibit the proliferation of cultured human SGH44 glioma cells in vitro, the viabilifies of MMC treated human SGH44 glioma cells were significantly lower than those in control group （ P 〈 0.05） ,the inhibitory effect was in a dose-dependent manner.The cell viabilifies of human SGH44 glioma cells treated with 2.50,5.00 and 10.00μmol·L ^- 1MMC for 4,8,16 and 32 h were significantly lower than those in control group（ P 〈 0.05）, the cytotoxicity effect was in a dose and time independent manner. When SGH44 glioma cells were treated with 0.075,0.15,0.3 and 1.2μmol· L^-1MMC for 12 h,the apoptosis/necrosis rates were significantly higher than those in control group（ P 〈 0.01 ）; decreased（ P 〈 0.01 ）. Conclusion MMC has cytotoxicity effect on cultured human SGH44 glioma cells in vitro, and can inhibit proliferation and induce apoptosis.