鼻咽癌组织中EBER_(_1)表达及其临床病理意义

EBER_(_1) Expression and its Clinicopathological Significance in Nasopharyngeal Carcinoma

目的探讨EBV与鼻咽癌发生发展关系及其临床病理意义、方法应用原位杂交技术检测76例鼻咽癌石蜡切片组织中EBER-1表达及分布状况。结果正常鼻咽粘膜上皮及其单纯性增生、鳞状化生上皮和头颈部其它肿瘤EBER＿1均阴性，鼻咽癌组织中EBER-1表达率为97．4%（74／76），且在转移灶中不丢失。鼻咽异型增生上皮亦可表达EBER＿1阳性信号（15／18）。结论EBV与鼻咽癌关系十分密切，EBV可能在鼻咽上皮向恶性转化过程中起重要作用。检测EBER-1对鼻咽癌诊断与鉴别诊断有重要价值。鼻咽上皮异型增生尤其是EBER-1间性者应认为是高危癌前病变，应密切随访监测可能有利于早期发现鼻咽癌。

Objective To study the relationship between EPstein-Bar virus (EBV) and the development of nasopharyngeal car cinoma (NPG) as well as the clinicopathological signifijcance of EBV encoded small RNA-1 (EBER- 1 ) expression.Methods The expression and distribution of EBER- 1 in paraffin-embedded tissue from 76 cases of NPC were studied by in situ hybridization.Results EBER.1 was not detected in normal tad simple hyperplasia of nasopharngeal epithelial cells, etaplasia of nasopha ryngeal mucosal epithelium and other tumors of head and neck. Of the 76 cases of NPC, 74 cases (97 .4% ) showed the exprse-sion of EBER- 1 that was still Present in metastatic NPC cells and dysplastic cells of paratumourous nasopharyngeal epithelium. Conchlsions There is a strong association between EBV and NRC. EBV may Play an important role in the pathogenesis of NPC, and EBER-1 expression is useful in the diagnosis and differential diagnosis of NPC.the dysPlastic cells of nasopharygeal epithelium especially those positive for EBER -1 can be regarded as a kind of precarcinomatous change. the measurement of EBFR- 1 may be helpflul in sereening early NPC in high risk populations.