山西和河南省部分地区人群流行性乙型脑炎病毒中和抗体水平调查

Neutralizing antibody level of Japanese encephalitis virus among healthy population in some areas in Shanxi province and Henan province

目的调查山西和河南省部分地区健康人群血清中流行性乙型脑炎(乙脑)病毒中和抗体水平。方法对采集的血清标本按1:10,1:20,1:40,1:80四个稀释度稀释后进行乙脑病毒中和试验,结果判定采用细胞病变观察方法并结合ELISA测定结果进行综合判定。结果调查结果显示,所测标本中乙脑病毒中和抗体保护率以临猗县最高(87.6%),其次是栾川县(58.2%)和万荣县(51.4%)。经χ2检验,临猗县与万荣县中和抗体保护率差异有统计学意义(χ2=42.812,P=0.000),临猗县的中和抗体保护率高于万荣县,栾川县与万荣县的中和抗体保护率差异无统计学意义(χ2=1.466,P=0.226)。两个年龄组(≤40岁和>40岁)中和抗体保护率比较显示,临猗县差异无统计学意义(χ2=0.057,P=0.811),万荣县差异有统计学意义(χ2=5.505,P=0.019),栾川县差异无统计学意义(χ2=2.129,P=0.145)。结论三个县健康人群乙脑病毒中和抗体保护率不同,这可能与当地乙脑的流行状况以及疫苗的接种情况有关。

Objective To investigate neutralizing antibody level of Japanese encephalitis virus among healthy people in some areas in Shanxi province and Henan province.Methods Serum samples collected were diluted by 1∶10,1∶20,1∶40,1∶80 to conduct neutralization test.The result interpretation will rely on both the cytopathic effect and the ELISA results.Results The results indicated that the neutralizing antibody protection rate in Linyi county（87.6%） was the highest,followed by Luanchun county（58.2%） and Wanrong county（51.4%）.By chi-square test,the difference on neutralizing antibody protection rate between Linyi county and Wanrong county had statistically significance（χ2=42.812,P=0.000）,while there was no statistically significant difference（χ2=1.466,P=0.226） between Luanchuan county and Wanrong county.There was statistically significant difference of neutralizing antibody protection rate between age group more than 40 years and age group less than 40 years in Wanrong county（χ2=5.505,P=0.019）,but no statistically significant differences were observed in Linyi county and Luanchuan county（χ2=0.057,P=0.811;χ2=2.129,P=0.145）.Conclusion The neutralizing antibody protection rate varied in 3 counties,which might relate with the local Japanese encephalitis prevalence and vaccination coverage.