云南粗茎秦艽不同采收期4种环烯醚萜苷类成分的测定

Determination of four secoiridoid glucosides in Gentiana crassicaulis during different harvest periods cultivated in Yunnan province

目的:采用高效液相色谱法,测定云南粗茎秦艽不同采收期根中龙胆苦苷、马钱酸、獐牙菜苦苷和獐牙菜苷4种环烯醚萜苷的含量。方法:采用Shimadzu C_(18)(150 mm×4.6 mm,5μm)色谱柱,以甲醇-0.4%磷酸水液梯度洗脱,流速1.0 mL·min^(-1),检测波长242 nm。结果:龙胆苦苷的含量远远高于其他3种环烯醚萜苷的含量,可作为药材质量评价的重要依据。无论是2年生还是3年生的栽培秦艽,7月份龙胆苦苷的含量均为最高值。结论:确定2年生秦艽的最佳采收期为7月份,而3年生秦艽的最佳采收期为12月份。

Objective：To determine the contents of gentiopicroside,loganic acid,swertiamarin and sweroside in Gentiana crassicaulis during different harvest periods cultivated in Yunnan province by HPLC method.Methods：The four components were separated by Shimadzu C_（18） column（150 mm×4.6 mm,5μm） with gradient mobile phase of methanol -0.4%phosphoric acid at the flow rate of 1.0 mL·min^-1 and the UV detection wavelength was 242 nm.Results：The contents of gentiopicroside was highest in July and could be used to evaluate the quality of the crud drug.Conclusion：Two and three years old roots of G.crassicaulis should be harvested in July and December, respectively.