HPLC法测定苦芪滴丸中苦参的5个生物碱含量

HPLC determination of 5 alkaloids of Radix Sophorae Flavescentis in Kuqi dropping pills

目的:采用高效液相色谱法对苦芪滴丸中苦参的5个单体生物碱(槐果碱、苦参碱、氧化槐果碱、槐定碱、氧化苦参碱)进行定量分析。方法:采用Inertsil NH_2(150 mm×4.6 mm,5μm)色谱柱,以乙腈(A)-无水乙醇(B)-3%磷酸水溶液(C)为流动相,梯度洗脱,流速1.0 mL·min^(-1),检测波长210 nm。结果:苦参的5个单体生物碱含量稳定。槐果碱、苦参碱、氧化槐果碱、槐定碱、氧化苦参碱进样量分别在0.14～0.49,0.38～1.30,0.06～0.20,0.05～0.18,0.13～0.44μg范围内呈良好的线性关系;r分别为0.9998,0.9997,0.9991,0.9991,0.9991。平均回收率(n=5)分别为98.2%,98.3%,97.7%,98.3%,98.9%。结论:方法可靠、实用,可用于苦芪滴丸的质量控制。

Objective：To develop an HPLC method for determination of 5 alkaloids of Radix Sophorae Flavescentis in Kuqi dropping pills.Methods：Inertsil NH2（150 mm×4.6 mm,5μm） was adopted;The mobile phase was acetonitrile （A） -absolute alcohol（B） -3%phosphonic acid water solution（C） with gradient elution at a flow rate of 1.0 mL·min^-1,and the detection wavelength was 210 nm.Results：The content of 5 alkaloids of Radix Sophorae Flavescentis was stable.The method has a good linearity in the ranges of 0.14 -0.49μg（r =0.9998） for sophocarpine, 0.38 - 1.30μg（r =0.9997） for matrine,0.06 -0.20μg（r =0.9991） for oxysophocarpine,0.05 -0.18μg（r = 0.9991） for sophoridine,and 0.13 - 0.44μg（r = 0.9991） for oxymatrine;The average recoveris were 98.2%,98.3%,97.7%,98.3%and 98.9%,respectively.Conclusion：This method is dependable,simple and practical,and may be used to control quality of Kuqi dropping pills.