紫外波长切换高效液相色谱法同时测定壮骨关节丸中7种有效成分的含量

HPLC simultaneous determination of seven active compounds in Zhuanggu Guanjie pills with switching UV wavelength

目的:采用紫外波长切换高效液相色谱法,建立同时测定壮骨关节丸中7种有效成分含量的方法。方法:色谱柱ZorbaxSB-C_(18)(4.6 mm×250 mm,5μm);流动相0.05%甲酸水溶液-乙腈,梯度洗脱;切换时间和检测波长:0～25 min,283 nm;25～30 min,365 nm;30～32 min,270 nm;32～37 min,246 nm;37～65 min,322 nm;柱温:40℃;流速:1.0 mL·min^(-1)。结果:柚皮苷、槲皮素、淫羊藿苷、补骨脂素、异补骨脂素、芒柄花素和蛇床子素在相应的浓度范围内与峰面积呈良好线性关系,相关系数均大于0.9997;平均回收率(n=6)在97.8%～104.3%之间,RSD均小于4.0%。结论:本方法简便快速、准确、精密度高、重复性好,适用于壮骨关节丸的质量控制。

Objective：To establish a method for simultaneous determination of seven active components in Zhuanggu Guanjie pills using switching UV wavelength.Method：The HPLC analysis performed on a Zorbax SB - C18column with the mobile phase composed of acetonitrile and 0.05%formic acid water in gradient elution.Detection wavelength was set at 283 nm in 0 - 25 min,365 nm in 25 - 30 min,270 nm in 30 - 32 min,246 nm in 32 - 37 min,322 nm in 37 -65 min.The column temperature was maintained at 40℃.The flow rate was fixed at 1.0 mL·min^-1.Results：Naringin,quercetin,icariin,psoralen,isopsoralen,formononetin and osthole showed good linearity over the tested ranges（r≥0.9997）.The average recoveries were ranged from 97.8 to 104.3%with RSDs≤4.0%.Conclusion：The developed method was used for quantification of seven active components in Zhuanggu Guanjie pills,which had advantage of accuracy,repeatability and simplicity.