HPLC法测定布格呋喃中的对映体

HPLC determination of enantiomer in buagafuran

目的:建立HPLC法测定布格呋喃中对映体的含量。方法:用Daicel Chiralcel OD手性色谱柱,正己烷为流动相,流速0.5 mL·min^(-1),柱温30℃,进样体积10μL,检测波长196 nm。结果:在上述色谱条件下,布格呋喃与对映体的分离度为4.24。对映体在1～20μg·mL^(-1)范围内与峰面积呈线性关系(r=0.9999),检测限为3 ng,定量限为10 ng。加样回收率为101.0%,RSD为1.4%(n=9)。布格呋喃中未检出对映体。结论:方法快速简便,实现了布格呋喃与对映体的手性分离,适用于布格呋喃中对映体的检查。

Objective：To establish an HPLC method for dermination of enantiomer as impurities in buagafuran. Methods：The Daicel Chiralcel OD column was used with n - hexane as mobile phase,flow rate was 0.5 mL·min^-1,column temperature was 30℃,injection volume was 10μL and wavelength was 196 nm.Results：Under this chromatographic condition,the resolution of buagafuran and its enantiomer was 4.24.Enantiomer showed a good linearity in the range of 1 - 20μg·mL^-1（r = 0.9999）.The limits of detection and quantitation of the enantiomer were 3 ng and 10 ng,respectively.The average recovery and RSD were 101.0%and 1.4%（n =9）.There was no enantiomer being detected in buagafuran.Conclusions：The method is rapid,simple and can be used for separation and determination of enantiomer in buagafuran.