蔬菜中百草枯的高效液相色谱测定法

Determination of Paraquat in Vegetables by High Performance Liquid Chromatography

目的建立蔬菜中百草枯残留量的高效液相色谱-紫外检测器测定方法。方法选用AgilentTC-C18色谱柱(4.6×250mm,5μm),检测波长257nm,以10mmol/L庚烷磺酸钠(用磷酸调pH=3.0)-甲醇(50+50)作为流动相,柱流量1.0ml/min,采用高效液相色谱法检测蔬菜中百草枯含量。结果该方法的线性范围为0.00～10.0μg/ml,R2=0.9994,RSD为0.75%～3.65%,回收率为96.1%～104.0%,检出限为0.03μg/ml。结论该方法简单、快速、准确度高,适用于蔬菜中百草枯残留量的测定。

Objective To establish a method for the determination of paraquat in vegetables by high performance liquid chromatography（HPLC）. Methods The HPLC separation was conducted by using an Agilent TC-C18 column （4.6 mmx250 mm,5μm）. The detection wavelength was 257 nm. The eluent is the mixture of 10 mmol/L sodium heptanesulfonate and methanol （50＋50） with the flow rate of 1.0 mt/min. Results The hnearity of the method ranged 0.00-10.0 μg/ml. The concemration of paraquat showed a good linear relationship （R2=0.999 4）, the RSDs were 0.75%-3.65%, the rates of recovery were 96.1%-104.0%, and the limit of detection was 0.03 μg/ml. Conclusion This simple and accurate method is applicable to the determination of paraquat in vegetables.