环境卷烟烟雾对大鼠血脂水平及脂代谢酶的影响

Effects of Environmental Tobacco Smoke on Serum Lipid and the Lipases of Serum and Liver and Lung in Rats

目的通过建立环境卷烟烟雾(environmental cigarette smoke,ETS)染毒模型,观察ETS对大鼠血脂水平、体内脂蛋白脂肪酶(LPL)和肝脂肪酶(HL)活力的影响。方法将20只健康清洁级SD大鼠随机分为吸烟组和对照组,每组10只,雌雄各半。吸烟组每天染毒2次,每次点燃卷烟6支,染毒60min,每周染毒6d,共染毒8周。测定大鼠血清中甘油三酯(TG)、胆固醇(TC)、高密度脂蛋白(HDL)及低密度脂蛋白(LDL)的水平和血清、肝脏和肺脏中的LPL和HL活力。结果吸烟组与对照组大鼠体重间比较,差异无统计学意义。与对照组相比,吸烟组大鼠血清TG较高,HDL较低,差异均有统计学意义(P<0.05);TC与LDL也有升高趋势,差异均无统计学意义。吸烟组肝脏和肺脏的LPL、HL活力以及血清HL活力低于对照组,差异均有统计学意义(P<0.05)。结论 ETS可引起大鼠体内脂肪代谢酶活力降低,导致血脂代谢紊乱。

Objective To study the effects of environmental tobacco smoke （ETS） on the levels of serum lipids and the activities of LPL and HL in rats. Methods A total of 20 SD rats （10 males, 10 females） were randomly divided into following two groups： smoking group and control group （10 rats in each）. The rats in smoking group were exposed to ETS 2 times a day for 8 weeks （inhaled ETS within 60 minutes each time, and 6 days a week）.The levels of serum lipids and the activities of LPL and HL in serum, lung and liver were measured respectively. Results There was no statistical significance between smoking group and control group in terms of body weight after being exposed to ETS for 8 weeks （P〉0.05）. The smoking group had a higher TG level and a lower HDL-C level than the control one with statistical significance（P〈0.05）. Though the levels of TC and LDL were increased in smoking group, there was no statistical significance （P〉0.05）. The HL activities in serum,lung and liver of smoking group were lower than those of control group （P〈0.05）. The smoking group had lower LPL activities in lung and liver than the control group with statistical significance （P〈0.05）. Conclusion ETS can reduce the activities of HL and LPL of blood,lung and liver, and cause hyperlipidemia in rats.