防晒化妆品中5种紫外吸收剂的超高效液相色谱测定法

Determination of Five Kinds of Ultraviolet Absorbents in Cosmetics by Ultra Performance Liquid Chromatography

目的建立防晒化妆品中2-羟基-4-甲氧基二苯酮、水杨酸苯酯、对二甲基氨基苯甲酸-2-乙基己酯、2,2,4,4-四羟基二苯甲酮、2-羟基-4-甲氧基二苯甲酮-5-磺酸5种紫外吸收剂同时测定的超高效液相色谱(UPLC)法。方法样品以甲醇为溶剂,经超声提取,以乙腈:水=70:30(V/V)为流动相进行分离,流量为0.3ml/min,检测波长310nm。结果在0～25mg/L的线性范围内,5种紫外吸收剂所得的回归方程均呈较好的线性关系,相关系数>0.999,检出限为4～6mg/L,最低检出浓度为0.01%～0.015%。该方法的平均回收率为98.35%～102.75%,RSD为0.44%～1.73%。结论该方法简便、准确、快速,适用于同时测定防晒化妆品中5种紫外吸收剂的含量。

Objective To establish an ultra performance liquid chromatography （UPLC） method for simultaneous determination of five kinds of ultraviolet absorbents in cosmetics, benzophenone-4 and benzophenone-5, etc. Methods The cosmetic samples were extracted with methanol, using acetonitrile/water=70：30（ V/V ）as mobile phase at the flow rate of 0.3 ml/min and detected at the wavelength of 310 nm. Results There was a good linear relationship over the range of 0-25 mg/L of five ultra violet absorbents,r〉0.999,the limits of detection of five ultraviolet absorbents were 4 to 6 mg/L and the minimum detectable concentration was 0.01%-0.015%. The rates of recovery for this method ranged from 98.35% to 102.75% and the relative standard deviations of this method were 0.44% to 1.73%. Conclusion The method is simple,rapid,accurate and applicable to simultaneous determination of the five ultraviolet absorbents in cosmetics.