植入血管束的血管化人工神经导管对受损神经功能恢复影响的实验研究

Effect of vascularized PGLA conduits on functional recovery of injured nerves: An experimental study

目的:研究植入血管束的血管化人工神经导管修复SD大鼠长段坐骨神经缺损对神经功能恢复的影响。方法:将18只成年雌性SD大鼠制成14mm的大鼠坐骨神经缺损模型后,随机分为3组(每组12条神经),分别采用不同的修复方法。A组:自体神经移植组(自体组);B组:普通PGLA神经导管移植组(导管组);C组:植入自体血管束的普通PGLA神经导管移植组(血管化导管组)。观察术后大鼠后肢皮肤溃疡面积;检测术后6周、12周时步态变化和肌电图。结果:术后各组SD大鼠均出现后肢溃疡,血管化导管组SD大鼠后肢溃疡愈合较导管组早2周。血管化导管组步态检测SFI明显优于导管组,与自体神经移植组无明显差异。肌电图检测表明血管化导管组无论是神经传导速度,还是动作电位振幅均明显大于导管组(P<0.05),与自体神经移植组无明显差异(P>0.05)。结论:植入血管束的血管化人工神经导管能有效地促进受损神经的功能恢复。

Objective： To evaluate the effects of repairing nerve defects with an artificial nerve conduit, which was vascularized by implantation of vascular bundle. Methods： The animals were divided randomly into three groups and used the different repairing materials. Group A： autogenous never transfer group; Group B： PGLA conduits transfer group; Group C：PGLA conduits with implanted autogenous vessels transfer group. Foot ulceration and walking pattern were observed every two weeks after operation. In addition, electrophysiological paraments were carried out at 6th and 12th week after operation. Results： The time of ulcer heal in group C is earlier than in group B. The test of electrophysiological paraments edicates that group C is better than group B in nerve conduction velocity and in action-electro-amplitude. Conclusions： Vascularized PGLA nerve conduit by implantation of vascular bundle can promote nerve regeneration and the nerve function repair.