游离脂肪酸对人牙周膜成纤维细胞增殖的影响(英文)

Effect of free fatty acids on proliferation of human periodontal ligament fibroblasts

目的:探讨游离脂肪酸(FFAs)对人牙周膜成纤维细胞增殖的影响,研究游离脂肪酸在代谢综合征患者牙周病发病机制中的作用。方法:选用在牙周组织修复中起主要作用的人牙周膜成纤维细胞进行体外培养,对照组加入不含胎牛血清的DMEM,实验组分别加入不同浓度的游离脂肪酸进行刺激,在刺激24h-72h后,采用四甲基偶氮唑蓝比色(MTT)法检测人牙周膜成纤维细胞的增殖情况。结果:与对照组相比,游离脂肪酸可以抑制人牙周膜成纤维细胞的生长增殖(P<0.01),并且这种抑制作用具有浓度和时间依赖性,以培养72h后抑制作用最为明显(P<0.01)。结论:游离脂肪酸可以抑制牙周膜成纤维细胞的增殖,降低代谢综合征患者牙周组织的的修复能力,从而导致或加重牙周病的发生或发展。

Objective： To investigate the effect of free fatty acids （FFAs） on proliferation of human periodontal ligament fibroblasts （hPDLFs） in vitro and to study the roles of free fatty acids in the mechanism between the metabolic syndrome （MetS） and periodontitis. Methods： HPDLFs were cultured in virto which played vital role in the periodontium tissue repaired. HPDLFs were deal with various concentrations of FFAs for 24h-72h and fibroblast proliferation was measured by colorimetric3[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide （MTT） assay. Results： FFAs inhibited the proliferation of hPDLFs in a dose-and time-dependent manner with MTT assay （P〈0.01 vs. control respectively）, the inhibition rate is the best significantly after cultured 72h （P〈0.01）. Conclusion： FFAs inhibits the proliferation ofhPDLFs in vitro and which lead to the delayed repare ofthe periodontal tissue in obeses patients.