乙型肝炎患者血清XCL1、IFN-γ与T细胞亚群的关系及临床意义

The Serum XCL1, IFN-γ and T Cells in Patients with Hepatitis B

目的:探讨XCL1、γ干扰素的浓度变化与乙型肝炎慢性化的关系。方法:采用酶联免疫吸附法(ELISA)检测血清XCL1、IFN-γ的浓度;流式细胞仪检测T淋巴细胞亚群;时间分辨荧光分析仪检测乙型肝炎抗原/抗体半定量指标;全自动生化仪检测肝功能。结果:(1)随着肝脏炎症程度的加重,血清中XCL1的含量逐渐增加。HBeAg+组与HBeAg-组比较,HBeAg+组血清XCL1浓度显著高于HBeAg-组(P<0.05);HBeAg-组血清IFN-γ浓度显著高于HBeAg+组(P<0.05)。(2)血清XCL1浓度与CD4+T细胞含量呈负相关(r=-0.336,P=0.017),IFN-γ浓度与CD8+T细胞含量呈正相关(r=0.319,P=0.024)。结论:γ干扰素通过影响XCL1的表达促进乙型肝炎慢性化的形成与发展,XCL1参与了乙型肝炎慢性化进程。

Objective： To explore the clinical significance and changes of serum level of XCL1、IFN-γ and the expression of T-cell subset in HBV-infected patients. Methods： The serum concentration of XCL1、IFN-γ were detected by enzyme linked immunosorbent assay （ELISA）; Peripheral blood T-cell subsets were detected by flow cytometry （FCM）; liver function was assayed by automatic biochemistry analyzer; hepatitis B antigen / antibody semi-quantitative index was detected by Time-resolved fluorescence analyzer. Results： （1） As the severity of liver inflammation, serum levels of XCL1 increasing.Between the group of HBeAg＋ and HBeAgSerum concentrations of XCL1 and IFN-γwere significantly different.（2） Serum concentrations of XCL1 and CD4＋ T cell were Negative related,on the other hand serum concentrations of IFN-γand CD8＋ T cell were positively related. Conclusion： IFN-γ expression by influencing XCL1 in the promotion and development of hepatitis B chronic pass.