造血干细胞向淋巴系祖细胞增殖分化过程中HOXC6基因表达的研究

The study of HOXC6 gene expression in the course of proliferation and differentiation from hematopoietic stem cell to lymphoid progenitor cell

目的探讨人脐血造血干细胞(HSC)向淋巴系祖细胞增殖分化过程中HOXC6基因表达的情况及全反式维甲酸(ATRA)对HOXC6基因表达的影响。方法采用造血干/祖细胞体外培养技术,以ATRA持续诱导人脐血造血干细胞,观察人脐血HSC经植物血凝素(PHA-M)诱导后,在培养过程第3、7和12天的淋巴系祖细胞集落生成情况;采用实时荧光定量PCR技术检测人脐血造血干细胞向淋巴系祖细胞增殖分化过程中HOXC6基因的表达水平。结果人脐血造血干细胞向淋巴系祖细胞增殖分化过程中,HOXC6基因表达呈现时间规律性。结论HOXC6基因在增殖分化的第7天表达最强烈,第12天表达明显降低,提示HOXC6基因与人脐血造血干细胞向淋巴系祖细胞增殖分化过程密切相关,可能是其调控基因之一。ATRA能显著上调HOXC6基因的表达。

Objective To explore homeobox （HOX） C6 gene expression in the course of proliferation and differentiation from the human umbilical cord blood hematopoietic stem cell （HSC） to lymphoid progenitor cell, and observe the influence of all-trans retinoic acid （ATRA） on HOXC6 gene expression. Methods The colony culture of hematopoietic stem/progenitor cells in vitro was adopted to observe colony cell growth of lymphoid progenitor cell on the 3rd, 7th arid 12th day in the course of proliferation and differentiation from HSC to lymphoid progenitor cell induced by ATRA and phytohemagglutinin （PHA-M）. The expression levels of HOXC6 was detected by fluorescence quantitative real time polymerize chain reaction （FQ-RT-PCR）. Relative amount of RNA expression （2- △△ct） was used to indicate the HOXC6 gene expression in the way of x± s. Results HOXC6 gene expression presented time regularity. HOXC6 gene expression was the highest on the 7th day and significantly reduced on the 12＇h day from HSC to lymphoid progenitor cell, which was consistent with the colony growth condition of lymphoid progenitor cell. Compared with control, ATRA up-regulated HOXC6 gene expression. Conclusion HOXC6 gene expression presented time regularity, which showed that there was a positive relationship between HOXC6 gene and the course of differentiatiofi and proliferation. ATRA can up-regulate the expression of HOXC6 gene.