摘要
以铁卟啉（Ｆｅ－ＴＰＰＳ４）作为过氧化物模拟酶，并以该酶催化过氧化氢氧化扑热息痛的荧光反应为基础，建立了用荧光度法测定速效伤风胶囊中扑热息痛含量的新方法．激发和发射波长分别为３１１．２ｎｍ与４３２．３ｎｍ，线性范围是０～８．０μｇ／ｍｌ（ｒ＝０．９９７０）和１２．０～２２．０μｇ／ｍｌ（ｒ＝０．９９９８）．该方法用于药物样品的测定．
Based on the catalytic fluorescence reaction of mimetic peroxidase (Fe TPPS 4) and paracetamol with hydrogen peroxidase, a new method for the determination of paracetamol in drug by fluorescence spectrophotomety is provided. The exitation and emission wave lengths are 311.2nm and 432.3nm, respectively. The linear ranges are 0～8.0μg/ml( r =0.9970)and 12.0～22.0μg/ml ( r =0.9998).The method has been applied to determination of the sample with a satisfactory result.
出处
《郑州大学学报（自然科学版）》
CAS
1998年第4期64-67,共4页
Journal of Zhengzhou University (Natural Science)
基金
河南省教委自然科学基金
关键词
过氧化物模拟
酶催化荧光法
扑热息痛
测定
mimetic peroxidase
paracetamol
catalytic fluorescence spectrophotometry
