长爪沙鼠对0.0025%氯敌鼠钠盐毒饵的致死食毒期检测

The lethal feeding period of 0.0025% chlorophacinone in Meriones unguiculatus

目的测定长爪沙鼠对0.0025%氯敌鼠钠盐毒饵的致死食毒期(LFP)。方法采用EPPO(欧洲及地中海植物保护组织)制定的基于LFP抗性检测方法。结果长爪沙鼠最低致死摄药量为6.3510mg/kg,最高存活摄药量为13.0230mg/kg。食毒期与其对应死亡率雌雄间差异有统计学意义(P=0.036),其食毒期-死亡率Probit模型分别为Probit(p)=-2.65195+0.79817x(P=0.901)和Probi(tp)=-5.62058+2.16045x(P=0.999)。对于LFP50、LFP98和LFP99及其95%置信限(CI),雌性长爪沙鼠分别为3.32253d(2.74004～3.95239)、5.89558d(4.93949～8.32331)和6.23711d(5.18450～8.95040),雄性长爪沙鼠分别为2.60159d(2.21062～2.98934)、3.55220d(3.11584～5.02447)和3.67838d(3.20401～5.32658)。结论用0.0025%氯敌鼠钠盐毒饵对长爪沙鼠进行LFP抗性检测时,LFP98的95%CI上限取整数天数作为抗药性检测标准,摄食期存活雌鼠为8d,雄鼠为5d;若用LFP99的95%CI上限取整数天数作为抗药性检测标准,摄食期存活雌鼠为9d,雄鼠为5d。

Objective To determine the lethal feeding period （LFP） of 0.0025% Chlorophacinone baits in Meriones unguiculatus. Methods A LFP-resistance-based detection method developed by the EPPO （European and Mediterranean Plant Protection Organization） was adopted. Results The minimum lethal dose was 6.3510 mg/kg for M. unguiculatus,the highest survival dose 13.0230 mg/kg. Statistically significant differences were observed in the feeding period and its corresponding mortalities between male and female groups （P=0.036）,the Probit model （feeding period versus mortality） being Probit （p）=-2.651 95＋0.798 17x （P=0.901）,and Probit（p）=-5.620 58＋2.160 45x （P=0.999）,respectively. The LFP50,LFP98,LFP99 and their 95% confidence limits（CI） were 3.322 53 d（2.740 04-3.952 39）,5.895 58 d（4.939 49-8.323 31）,and 6.237 11 d （5.184 50-8.950 40） in the female M. unguiculatus; and 2.601 59 d （2.210 62-2.989 34）,3.552 20 d （3.115 84-5.024 47） and 3.678 38 d （3.204 01-5.326 58） in the male M. unguiculatus. Conclusion In LFP resistance detection using 0.0025% Chlorophacinone baits in M. unguiculatus,the 95%CI upper limit of LFP98,an integer number of days when applied as the drug-resistant testing standards,was 8 d for female rats and 5 d for males that survived in the feeding period; on the other hand,the 95%CI upper limit of LFP99,as an alternative,was 9 d for surviving female rats and 5 d for surviving males in the feeding period.