猪脑栓塞动物模型的建立及评价

A Pig Model of Acute Experimental Embolic Stroke

取100μl正常人血浆在体外制成0.5～0.8mm大小的血栓颗粒,自猪颈内动脉注入颅内,用DSA观察栓塞部位,24h后作病理检查.结果:猪的颈内动物入颅前先形成网状结构,再合成一主干分出颅内各分支,血栓均受阻于网前或网中.栓塞成功率100%,6h后脑血管造影栓子未溶解,病理示5例出现脑梗死.结论:该模型栓塞部位固定、成功率高,能用DSA作为可靠的观察指标,可用于评价溶栓疗效.

A hundred μl normal human plasma was allowed to clot by extravascular coagulation. The clot was divided into 0.5~0. 8 mm segments, and were infected into one internal carotid artery of a pig. The vascular occlusion was observed by digital subtraction arteriography (DSA). Twenty four hours after embolization, the pigs were killed. The brains were cut into 0. 5 cm-thick coronal section and stained with triphenyltetrazolium chride. The rates of distal internal carotid arterial obstruction was 100%. Six hours after embolization, none of the arteriograms of the embolized internal arteries showed thrombus dissolution. 5 of 6 animals showed cerebral infarction. This model was highly reproducible concerning embolic vessel obstruction, and could observe vascular occlusion by DSA. It was suitable for the study of thrombolytic agents.