复原胶囊对脓毒症大鼠肾组织HIF-1α表达的影响

Effect of Fuyuan Capsule on the Expression of HIF-1α Kidney of Sepsis Rats

目的:研究复原胶囊对脓毒症大鼠肾组织缺氧诱导因子-1α(HIF-1α)mRNA表达的影响。方法:雄性SD大鼠随机分为假手术、模型及复原胶囊高、低剂量组,每组4只。复原胶囊组大鼠连续4d灌胃后行盲肠结扎穿孔(CLP)手术。各组复制模型后分别于3、6、12、24h行RT-PCR检测肾组织HIF-1α的表达;酶联免疫吸附法检测血清白介素-6(IL-6)水平;心脏取血检测血清肾功能;HE染色镜下观察肾组织病理学变化。结果:与假手术组比较,模型组HIF-1αmRNA表达3h时逐渐升高,24h达高峰(P<0.05);与模型组比较,复原胶囊高、低剂量组6、12、24h时HIF-1αmRNA表达显著降低(P<0.05);复原胶囊高、低剂量组BUN显著降低(P<0.05);HE染色显示复原胶囊组损伤较轻。结论:复原胶囊对脓毒症大鼠肾损伤的保护作用可能与调控HIF-1α的表达有关。

OBJECTIVE：To explore the effect of Fuyuan capsule on the mRNA expression of HIF-1α in kidney of sepsis rats. METHODS：Male SD rats were randomly divided into sham group,model group and Fuyuan capsule group （low dose and high dose） with 4 rats in each group. CLP operation was done in Fuyuan capsule groups after four days of intragastric administration. RT-PCR testing was applied to determined the expression of HIF-1a in 3 h,6 h,12 h and 24 h after model induction. The level of IL-6 was measured by ELISA method. Blood of heart was sampled for detection of renal function. HE staining was used to observe pathological change of kidney. RESULTS：As compared with sham group,the mRNA expression of HIF-1α increased gradually within 3 h and reached peak at 24 h（P〈0.05）. The mRNA expression of HIF-1α in Fuyuan capsule groups decreased strikingly at 6 h,12 h and 24 h,compared with model group（P〈0.05）. The level of blood urea nitrogen （BUN） in Fuyuan capsule groups reduced strikingly（P〈0.05）. HE staining showed there was little damage in Fuyuan casuple groups. CONCLUSION：The protection effect of Fuyuan capsule on kidney injure in sepsis rats maybe associated with the regulation of HIF-1α expression.