慢性癫癎大鼠脑组织生长抑素mRNA表达变化及糖皮质激素的作用

The Changes of Somatostatin mRNA in Chronic Epileptic Rats and Effects of Glucocorticoid Hormones on It

为探讨生长抑素在癫（疒间）发病中的作用及糖皮质激素对慢性癫（疒间）大鼠脑组织生长抑素mRNA(SOMmRNA)表达的影响.应用原位杂交组织化学方法研究了3组大鼠海马回、齿状回SOMmRNA表达变化,并用显微图像分析仪进行检测分析.结果表明,慢性癫(疒间)大鼠海马回、齿状回SOMmRNA胞体数量、胞体截面积均明显高于对照组,胞体灰度值均明显低于对照,应用地塞米松(Dex)后上述改变不明显.提示:SOM基因的活化参与了癫(疒间)的发病,Dex的抗(疒间)作用与其抑制SOM基因的活化有关.

In order to explore the effects of glucocorticoid hormones of the expression of somatostatin mRNA(SOMmRNA) in hippocampus and the pathogenesis of somatostatin in epilepsy, an in situ hybridization and image analyses study were made on the expression of SOMmRNA in hippocampus in 3 groups. Results showed a markedly increased of the number, the sectional area of SOMmRNA-positive cell bodies, and a markedly decrease of gray value of SOMmRNA-positive cell bodies in the hippocampus gyrus, dentate gyrus in the chronic epileptic groups as compared with the control groups. Dexamethasone (Dex) abolished those changes. It is indicated activation of SOM gene involved in the pathogenesis of epilepsy, antiepilepsy ability of glucocorticoid hormones might have relation to somatostatin.