冻贮大鼠甲状腺组织自体移植的计量形态学研究

MORPHMETRIC STUDIES ON THE CRYOPRESERVED RAT THYROID AUTOGRAFTS

应用计量形态学的方法,对大鼠自体移植的新鲜和冷冻的甲状腺组织进行了光镜和电镜观察.结果表明,26只大鼠行自体移植的新鲜和冷冻的甲状腺组织,4周末均存活且生长良好.冷冻保存移植物的面积及其滤泡的平均直径,均明显小于新鲜移植物(P<0.01).但在超微结构水平上,冷冻及新鲜移植物的滤泡上皮细胞,其线粒体和粗面内质网的体密度和面密度无显著差别(P>0.05).提示冷冻后移植物的存活细胞数少于新鲜移植物,但电镜下二者单个滤泡的主要细胞器的大小和含量并无差别,说明生长4周的冷冻移植物的滤泡上皮细胞结构已完全复原,从结构上已看不出冷冻损伤的影响.

Cryopreserved thyroid tissues of Wistar rats were autotransplanted into the renal subcapsular space. Both the cryopreserved and fresh grafts were found to have survived in all the 26 animals. The areas and diameters of fresh grafts were siqnificantly larger than those of cryopreserved grafts(P<0.01). There was no obvious difference between the two kinds of autografts in volume density and membrane surface density of mitochondria and rough endoplasmic reticulum (P>0.05).