HBeAg对慢性乙型肝炎孕妇胎盘组织TLR4表达的作用研究

The effect of HBeAg on TLR4 expression in placenta of pregnant women with chronic hepatitis B

目的探讨HBeAg对慢性乙肝孕妇胎盘组织Toll样受体4(TLR4)表达的作用,及TLR4表达与胎盘持续感染HBV的关系。方法选取22例HBeAg阳性和24例HBeAg阴性慢性乙肝孕妇,及正常孕妇25例。以实时荧光定量RT-PCR(qRT-PCR)和免疫组化法检测胎盘组织中TLR4的表达,并采用实时荧光定量PCR和微粒子酶免疫分析法(MEIA)分别检测其血清HBV DNA和HBeAg水平。结果HBeAg阳性孕妇胎盘组织中TLR4mRNA的表达水平明显低于正常对照组和HBeAg阴性组(P<0.01),但与正常对照组相比,其血清HBeAg和HBV DNA水平则显著升高(P<0.01);胎盘组织中TLR4 mRNA表达与HBeAg水平呈负相关,而与血清HBV DNA水平无显著相关性。结论HBeAg对胎盘组织TLR4表达起负调节作用,胎盘组织中TLR4的表达与其持续感染HBV有一定关系。

Objective：To explore the effect of HBeAg on TLR4 expression in placenta of pregnant women with chronic hepatitis B（CHB）,and the relationship of TLR4 expression and HBV persistent infection in placenta.Methods：To choose 22 HBeAg-positive and 24 HBeAg-negative CHB pregnant women,and 25 normal pregnant women.To detecte the expression of TLR4 mRNA in placentas by real-time fluorescence quantitative RT-PCR（qRT-PCR） and immunohistochemical method.To assay the level of HBV DNA and HBeAg by real-time fluorescence quantitative PCR（FQ-PCR） and microparticle enzyme immunoassay（MEIA）,respectively.Results：The level of TLR4 was lower in HBeAg-positive CHB pregnant women than that in HBeAg-positive CHB and normal pregnan women（P0.01）.But compared with HBeAg-positive CHB and normal pregnan women group,their levels of HBV DNA and HBeAg in serum rise significantly（P0.01）.There was a negative relation between expressions of TLR4 mRNA in placentas and levels of HBeAg in serum,but no relation between TLR4 and HBV DNA.Conclusions：HBeAg play a negative role in the expression of TLR4 in placentas,and the expression of TLR4 has a association with HBV persistent infection in placenta of pregnant women.