眼镜蛇神经毒素配伍咖啡因镇痛作用研究

Study on the analgesia of Chinese cobra-neurotoxin compatibility with caffeine

目的:研究眼镜蛇神经毒素(α-CNT)配伍咖啡因(CAF)镇痛效果。方法:小鼠扭体镇痛试验:各组动物给药30min后,立即注射0.6%醋酸0.2mL/只,记录15min内各组动物扭体次数;小鼠热板镇痛试验:分别测定各组动物给药后15,30min、1,2,3,4,5,6,8,10h,在55℃热板上痛反应时间,以小鼠舔足、跳跃、抬足为指标,记录各组动物痛反应时间。结果:眼镜蛇神经毒素配伍咖啡因能明显抑制化学刺激引起小鼠的痛反应;能明显延长小鼠热刺激引起的痛反应时间。结论:眼镜蛇神经毒素配伍咖啡因[(α-CNT-CAF(眼镜蛇神经毒素配伍咖啡因)]对疼痛动物模型有明显的镇痛效果。

OBJECTIVE To study the analgesic effect of Chinese cobraneurotoxin compatibility with caffeine. METHODS The analgesic effect was tested by using writhing modelsin mice. After 30 min after giving the mice with corresponding drugs in every group,0. 6% HAc 0. 2 mL/20 g was injected and the turnning round frequencies of the mice were recorded group in 15 rain. The analgesic experiment of the heated plate in mice was used to determine the time that the mice felt pain on the heated plate （T=55 ℃） after corresponding drugs in 15min,30min, 1,2,3,4,5,6,8,10 h. RESULTS α-CNT -CAF has restraining effect to pain caused by chemical irritation in mice, obviousely prolong the time of pain reaction caused by heating irritation. CONCLUSION α- CNT CAF possesses analgesic effects.