期刊文献+

丁酸钠对卵巢癌细胞侵袭能力的抑制作用及其与血管内皮生长因子表达的关系 被引量:2

Inhibitory effect of sodium butyrate on invasion of human ovarian cancer cell line CAOV3 and its effects on VEGF expression
原文传递
导出
摘要 目的:探讨组蛋白去乙酰基酶抑制剂丁酸钠对卵巢癌细胞株CAOV3侵袭能力的抑制作用及其对血管内皮生长因子(VEGF)表达的影响。方法:用不同浓度的丁酸钠处理CAOV3细胞,采用锥虫蓝活细胞拒染法检测癌细胞体外生长活性,Transwell小室观察其对细胞运动及侵袭能力的影响,利用RT-PCR技术检测CAOV3细胞中VEGF mRNA表达的变化。结果:丁酸钠抑制CAOV3细胞的生长,并呈剂量依赖性(P<0.05);其对CAOV3细胞的运动及侵袭能力均有明显抑制作用(P<0.05);并且能显著降低VEGF mRNA的表达(P<0.05)。结论:丁酸钠能有效抑制卵巢癌细胞株CAOV3的侵袭转移,可能与降低VEGF的表达有关。 ORJECTIVE To examine the inhibitory effect of sodium butyrate on the invasion of human ovarian cancer cell line CAOV3 and its effects on VEGF expression. METHODS The CAOV3 cells were exposed to various concentration of sodium butyrate. The growth activities of cancer cells were detected by trypan blue stain method. Transwell assay were used to evaluate the migration and invasion ability. RT-PCR was used to observe the change of VEGF mRNA expression. RF_SULTS Sodium butyrate could inhibit the growth of CAOV3 cell line, and this inhibition was in a dose-dependent pattern(P〈0. 05). And it could reduce abilities of migration and invasion in CAOV3 cell line in a dose-dependent pattern significantly(P〈0. 115). The VEGF mRNA expressions were down-regulated in sodium butyrate groups(P〈0. 05). CONCLUSlON Sodium butyrate can in- hibit the growth and invasion ability of ovarian cancer in vitro, probably via down-regulation of VEGF.
出处 《中国医院药学杂志》 CAS CSCD 北大核心 2010年第7期570-573,共4页 Chinese Journal of Hospital Pharmacy
关键词 丁酸钠 卵巢肿瘤 侵袭 血管内皮生长因子 sodium butyrate ovarian tumor invasion VEGF
  • 相关文献

参考文献10

  • 1Holger HS. Histone deacetylase inhibitors and cancer: from cell biology to the clinic[J]. Eu J Cell Biol,2005,84:109-121.
  • 2Marks PA, Miller T, Richon VM. Histone deacetylases[J]. Curr Opin Pharmacol,2003,3(4)=344 351.
  • 3Rodriguez Salvador J, Armas-Pineda C, Perezpena-Diazconti M,et al. Effect of sodium butyrate on pro-matrix metalloproteinase-9 and -2 differential secrelion in pediatric tumors and cell lines[J]. J Exp Clin Cancer Res,2005,24(3) :463-473.
  • 4Liu LT, Chang HC, Chiang LC,et al. Histone deacetylase inhibitor up-regulates RECK to inhibit MMP-2 activation and cancer cell invasion[J]. Cancer Res, 2003,63 (12) : 3069-3072.
  • 5Pulukuri SM, Gorantla B, Rao J S. Inhibition of histone deacetylase activity promotes invasion of human cancer cells through activation of urokinase plasminogen activator[J]. J Biol Chem, 2007,282(49) :35594-35603.
  • 6Belotti D, Calcagno C, Garofalo A, et al. Vascular endothelial growth factor stimulates organ-specific host matrix metalloproteinase-9 expression and ovarian cancer invasion[J]. Mol Cancer Res,2008,6(4):525-534.
  • 7Duncan TJ, Al-Attar A, Rolland P, et al. Vascular endothelial growth factor expression in ovarian cancer: a model for targeted use of novel therapies [J]. Clin Cancer Res,2008,14(10) : 3030-3035.
  • 8Hu Z,Fan C, Livasy C, He X, et al. A compact VEGF signature associated with distant metastases and poor outcomes[J]. BMC Med,2009,7:9.
  • 9Muhlethaler-Mottet A, Meier R, Flahaut M, et al. Complex molecular mechanisms cooperate to mediate histone deacetylase inhibitors anti-tumour activity in neuroblastoma cells[J]. Mol Cancer,2008,12(7) :55.
  • 10Heider U, Kaiser M, Sterz J, et al. Histone deacetylase inhibitors reduce VEGF production and induce growth suppression and apoptosis in human mantle cell lymphoma [J]. Eur J Haematol, 2006,76 ( 1 ) : 42-50.

同被引文献37

  • 1梁爱玲,侯敢,黄迪南.丁酸钠诱导MCF-7细胞凋亡的研究[J].现代肿瘤医学,2006,14(8):916-918. 被引量:3
  • 2沈蓉,于东红.丁酸钠抗肿瘤作用分子机制研究进展[J].蚌埠医学院学报,2007,32(2):237-239. 被引量:2
  • 3Hess-Stumpp H. Histone deacetylase inhibitors and cancer: from cell biology to the clinic [ J]. Eu J Cell Biol,2005,84 (2-3) :109 -121.
  • 4Marks PA, Xu WS. Histone deacetylase inhihitors: potential in cancer therapy[ J]. J Cell Biochem ,2009,107 (4) :600 - 608.
  • 5Jiang S,Dowdy SC,Meng XW, et al. Histone deacetylase inhibi- tots induce apoptosis in both Type I and Type lI endometrial cancer cells[J]. Gynecol Oncol,2007,105 (2) :493 - 500.
  • 6Chen S, Han YH, Zheng Y, et al. NDRG1 contributes to retinoic acid-induced differentiation of leukemic ceils [ J ]. Leuk Res, 2009,33(8) :1108 - 1113.
  • 7Le NT, Richardson DR. Iron chelators with high antiproliferative activity up-regulate the expression of a growth inhibitory and me- tastasis suppressor gene : a link between iron metabolism and pro- liferation [ J ]. Blood,2004,104 (9) :2967 - 2975.
  • 8Jemal A, Bray F, Center MM, et al. Global cancer stalistcs[ J]. CA Cancer J Clin,2011,61 (2) :69 -90.
  • 9Marks PA, Miller T, Richon VM. Histone deacetylases[J]. Curr Opin Pharmaco1,2003,3 (4) :344 - 351.
  • 10Glozak MA, Seto E. Histone deaeetylases and cancer[J] Oneo- gene ,2007,26 ( 37 ) :5420 - 5432.

引证文献2

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部