丁酸钠对卵巢癌细胞侵袭能力的抑制作用及其与血管内皮生长因子表达的关系

Inhibitory effect of sodium butyrate on invasion of human ovarian cancer cell line CAOV3 and its effects on VEGF expression

目的:探讨组蛋白去乙酰基酶抑制剂丁酸钠对卵巢癌细胞株CAOV3侵袭能力的抑制作用及其对血管内皮生长因子(VEGF)表达的影响。方法:用不同浓度的丁酸钠处理CAOV3细胞,采用锥虫蓝活细胞拒染法检测癌细胞体外生长活性,Transwell小室观察其对细胞运动及侵袭能力的影响,利用RT-PCR技术检测CAOV3细胞中VEGF mRNA表达的变化。结果:丁酸钠抑制CAOV3细胞的生长,并呈剂量依赖性(P<0.05);其对CAOV3细胞的运动及侵袭能力均有明显抑制作用(P<0.05);并且能显著降低VEGF mRNA的表达(P<0.05)。结论:丁酸钠能有效抑制卵巢癌细胞株CAOV3的侵袭转移,可能与降低VEGF的表达有关。

ORJECTIVE To examine the inhibitory effect of sodium butyrate on the invasion of human ovarian cancer cell line CAOV3 and its effects on VEGF expression. METHODS The CAOV3 cells were exposed to various concentration of sodium butyrate. The growth activities of cancer cells were detected by trypan blue stain method. Transwell assay were used to evaluate the migration and invasion ability. RT-PCR was used to observe the change of VEGF mRNA expression. RF_SULTS Sodium butyrate could inhibit the growth of CAOV3 cell line, and this inhibition was in a dose-dependent pattern（P〈0. 05）. And it could reduce abilities of migration and invasion in CAOV3 cell line in a dose-dependent pattern significantly（P〈0. 115）. The VEGF mRNA expressions were down-regulated in sodium butyrate groups（P〈0. 05）. CONCLUSlON Sodium butyrate can in- hibit the growth and invasion ability of ovarian cancer in vitro, probably via down-regulation of VEGF.