基质金属蛋白酶-2及9与子宫内膜腺癌临床及预后的关系

The expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 in endometrial adenocarcinoma and the relations of clinical and prognosis

目的探讨基质金属蛋白酶-2(MMP-2)和9(MMP-9)在正常子宫内膜组织、子宫内膜腺癌组织中的表达,及其与子宫内膜腺癌临床和预后的关系。方法2005年10月至2009年3月在中山大学附属中山医院采用免疫组织化学EnVision法,检测100例子宫内膜腺癌组织中MMP-2、MMP-9蛋白的表达,以30例正常子宫内膜组织作为对照组。结果MMP-2、MMP-9在正常子宫内膜增生期表达增加而在早中分泌期几乎无表达(P>0.05)。MMP-2、MMP-9蛋白在子宫内膜腺癌组织中的表达为74.0%和78.0%,显著高于正常子宫内膜组织(50.0%、56.7%)(P<0.05);随着子宫内膜腺癌临床分期、病理组织学分级的提高,MMP-2、MMP-9的表达也随之增强,且有统计学意义(P<0.05);在伴有淋巴结转移的子宫内膜腺癌组织中,两者表达(90.0%、60.0%)高于无淋巴结转移组(28.6%、23.8%)(P<0.05)。子宫内膜组织中MMP-2与MMP-9蛋白的表达呈正相关(r=0.545,P<0.001)。MMP-2和MMP-9的表达与患者生存率相关(P<0.05)。结论MMP-2、MMP-9在子宫内膜腺癌发生、发展过程中可能有促进作用。两者的表达与临床分期、组织学分级、淋巴转移有关,检测两者的表达可能有利于预测子宫内膜腺癌的预后。

Objective To study the relationship between the expression of matrix metalloproteinase （MMP）-2 and -9 in endometrial endometrial adenocarcinoma normal endometrial tissue and clinical and prognosis of the disease. Methods The expression of MMP-2 and MMP-9 were examined in 100 patients with endometrial adenocarcinoma Between October of 2005 and March of 2009 in Zhongshan Hospital Affihated to Sun Yat-sen University by immunohistochemistry （EnVision method）. We used 30 normal endometrial tissue as control. Results The normal endometrium mesenchymal and the epithelial cell may secrete MMPs,MMP-2,9,in the normal endometrium proliferative phase expression increases,but in the secretory phase nearly early does not have the expression （P0. 05）. The expression of MMP-2 and MMP-9 in endometrial adenocarcinoma was significantly stronger than that in normal endometrial （P0.05）.The expression of MMP-2 and MMP-9 in endometrial adenocarcinoma tissues was increased with advanced clinical stages and pathological grades （P0.05）. As compared to the patiens without lymph node metastasis,the expressions of MMP-2 and MMP-9 in endometrial carcinoma was significantly higher than that in patients with lymph node metastasis. The expression of MMP-2 and MMP-9 was positively correlated （r= 0.545,P 0.01）. The expression MMP-2 and MMP-9 was correlated with patient survival （P0.05）. Conclusion MMP-2 and MMP-9 might promote the development of endometrial adenocarcinoma. The expression of MMP-2 and MMP-9 in endometrial adenocarcinoma was related to the clinical stages,pathological grades,and lymph node metastasis and can be used to predict the prognosis of endometrial adenocarcinoma.