高迁移率蛋白A2在不同宫颈病变中的表达及意义

Expression and clinical significance of HMGA2 in cervical intraepithelial neoplasms and cervical cancer

目的探讨高迁移率蛋白A2（HMGA2）在不同宫颈病变组织中的表达及其临床意义,了解其与宫颈人乳头瘤病毒（HPV）感染的关系。方法免疫组织化学法检测无锡市第三人民医院2005至2007年30例正常宫颈组织、19例低级别上皮内瘤样病变宫颈组织、16例高级别上皮内瘤样病变宫颈组织、13例宫颈鳞癌组织中HMGA2蛋白的表达,原位杂交检测各组织中HPV感染的情况,分析HMGA2蛋白表达与HPV感染在宫颈病变组织中的相关性。结果正常宫颈组织、CINⅠ级、CINⅡ~Ⅲ级和宫颈浸润性鳞癌组织中,HMGA2蛋白的阳性表达率分别为26.67%、21.05%、68.75%和76.92%。CINI与正常宫颈组织阳性表达率比较,差异无统计学意义;CINⅡ~Ⅲ级、宫颈浸润性鳞癌组织与正常宫颈组织阳性表达率比较,差异有统计学意义（P=0.011,P=0.006）。宫颈高级别上皮内瘤样病变和宫颈癌组织具有较高的HPV感染率,HPV感染与否和HMGA2的表达两者之间未发现统计学相关性。结论HMGA2高表达于高级别宫颈上皮内瘤样病变组织和宫颈癌组织,这可能是宫颈病变的一个早期事件。HMGA2的表达与宫颈HPV感染两者没有相关性,HMGA2可能是宫颈恶性转化的内因之一。

Objective To investigate the expression and clinical significance of HMGA2 in cervical intraepithelial neoplasms and cervical cancer and the relationship with HPV. Methods Expression of HMGA2 protein were retrospectively studied by immunohistochemistry in 30 normal cervical tissues, 19 low grade of cervical intraepithelial neoplasms,16 high grade of cervical intraepithelial neoplasms, 13 cervical squamous cancer. HPV were detected with hybridization in situ. Results The expression of HMGA2 was 26.67%,21.05%,68.75% and 76.92% in normal cervical tissues,CINⅠ,CINⅡ~Ⅲ and cervical squamous cancer respectively （χ2=17.11,P0.001）. There was negative correlation between the expression of HMGA2 and HPV infection. Conclusion The abnormality of HMGA2 expression maybe an early event in transformation of cervical cancer. HMGA2 possibility is an intrinsic factor in the transformation for the negative correlation with HPV.