卵巢浆液性癌组织中Hugl-1和aPKC的表达及意义

Expression of Hugl-1 and aPKC in serous epithelial ovarian cancer

目的探讨原发性卵巢浆液性癌组织中Hugl-1和aPKC的表达及意义。方法选择浙江大学医学院附属妇产科医院2000年6月至2007年6月经手术切除的原发性卵巢浆液性癌组织79例,用免疫组化SP法检测Hugl-1和aPKC蛋白在其组织中的表达。同时检测Hugl-1和aPKC蛋白在25例正常卵巢组织,31例卵巢浆液性囊腺瘤,41例卵巢交界性浆液性肿瘤中的表达,并分析了Hugl-1和aPKC蛋白表达与卵巢癌临床病理参数间的关系。结果Hugl-1蛋白在卵巢浆液性癌组织、正常卵巢、浆液性囊腺瘤及交界性浆液性肿瘤中的强阳性表达率分别为12.7%、48%、58%、41.5%。癌组织中的表达显著低于其余3组,差异有统计学意义(P<0.001);aPKC蛋白在卵巢浆液性癌组织、正常卵巢、浆液性囊腺瘤及交界性浆液性肿瘤中的强阳性表达率分别为19%、0、0、0。癌组织中的表达显著高于其余3组,差异有统计学意义(P<0.001);Hugl-1和aPKC的表达与组织学分级、手术病理分期、淋巴结转移有一定的相关性(P<0.05)。Hugl-1和aPKC在卵巢癌组织中的表达不存在相关性(P>0.05)。结论Hugl-1和aPKC可能均参与了卵巢癌的发生与发展。

Objective To study the expression and significance of Hugl-1 and apical protein kinasec （aPKC） in serous epithelial ovarian cancer. Methods The expressions of Hugl-1 and aPKC protein in 25 normal epithelial ovarian tissues, 31 benign, 41 borderline and 79 malignant serous ovarian cancer tissues were detected with the method of immunohisto-chemistry. The relationship between the respective expression of Hugl-1 and aPKC in serous epithelial ovarian cancer and various clinicopathologic factors had been analyzed. Results Positive expressions of Hugl-1 in serous ovarian cancers, normal ,benign and borderline were 12.7% ,48% ,58% ,41.5% respectively. The expressions of Hugl-1 in serous ovarian cancers were significantly lower than those in normal, benign and borderline tissues（P 〈0. 001 ）. The expressions of aPKC in serous ovarian cancers normal ,benign and borderline were 19% ,0,0,0 respectively. The expressions of aPKC in serous ovarian cancers were significantly higher than those in other groups（P 〈0. 001 ）. The expressions of Hugl-1 and aPKC were respectively related to pathological grade, clinical stage and Lymph node metastasis. There wasn＇ t correlation between the expressions of the two proteins in serous ovarian cancer（P 〉 0. 05）. Conclusion The two proteins may be involved in the carcinogenesis of serous ovarian cancer.