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人胚胎干细胞向滋养层分化的研究进展

Studies on the differentiation of trophoblast from human embryonic stem cell
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摘要 哺乳动物胚胎发育产生的第一个细胞系的分离是内细胞团和滋养层的分离,不同哺乳动物之间胚胎干细胞向滋养层细胞分化不同,滋养层细胞对胚胎的植入、促进胚胎在子宫内的生存和生长至关重要.人胚胎干细胞为研究人类胚胎发育及向滋养层分化提供了一个独特的模型.人胚胎干细胞可以在实验室条件下保持无限期稳定的培养,用于最初胚胎和滋养外胚层发生的机制研究.目前人胚胎干细胞分化为滋养层细胞在体外可以通过自发分化、基因敲除、分离EB小体和BMP4诱导等几种途径实现.不同哺乳动物之间胚胎干细胞向滋养层分化机制,主要通过信号通路如BMP4,LIF等以及某些标志基因如OCT4,CDX2,Eomes等的变化调节.人胚胎干细胞向滋养层分化的研究为临床应用提供了一定的基础. The emergence of the inner cell and the trophoblast is the first step in cell lineage segregation of the developing mammalian embryos.Embryonic stem cells in different mammals are different to the trophoblast differentiation,the trophoblast is crucial for embryo implantation,as well as promotion of embryo survival and growth in the uterus.Human embryonic stem cells provide a unique model for studying human early embryonic development.As a model,the advantages of human embryonic stem cells are that they can maintained indefinitely in the laboratory and provide the ability to study the initial embryonic/trophectoderm transition.Human embryonic stem cells can be prompted to form trophoblast by self-differentiation,the transgenic knockdown of genes associated with pluripotency,separate of embryoid bodies(EB),BMP4 treatment of stem cells.Between different mammalian embryonic stem cells to the trophoblast differentiation in the signal pathway,such as BMP4,LIF,and so on,and certain marker genes such as OCT4,CDX2,Eomes have some similarities and differences.Human embryonic stem cells to the trophoblast differentiation of clinical research provides some basis.
出处 《中国科学:生命科学》 CSCD 北大核心 2010年第3期202-209,共8页 Scientia Sinica(Vitae)
基金 国家自然科学基金重点(批准号:30530760)项目资助
关键词 胚胎干细胞 滋养层细胞 BMP4 胚胎植入 胚状体 embryonic stem cell trophoblast BMP4 implantation embryoid body
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