红三叶总异黄酮对人增生前列腺组织基质细胞增殖和凋亡的影响

Red clover isoflavones inhibit the proliferation and promote the apoptosis of benign prostatic hyperplasia stromal cells

目的:评价红三叶总异黄酮对人增生前列腺组织基质细胞增殖和凋亡的影响。方法:采用浓度为12.5、25、50、100μg/ml的红三叶总异黄酮溶液处理前列腺基质细胞,并设立PBS空白对照组,DMSO阴性对照组和浓度为12.5、25.0、50.0、100.0μg/ml的非那雄胺溶液为阳性对照组。MTT法测定红三叶总异黄酮对细胞增殖的影响;AnnexinVFITC/PI双染色法流式细胞术分析红三叶总异黄酮对细胞凋亡的作用。结果:当红三叶总异黄酮浓度达到25.0μg/ml时,其对人前列腺增生组织基质细胞的增殖抑制率为18.86%,与空白对照组(5.17%)相比差异有显著性(P<0.05);且随着药物浓度的增加,抑制作用也愈趋明显。与非那雄胺阳性对照组相比,当浓度达到50.0μg/ml时,红三叶总异黄酮实验组对人前列腺增生组织基质细胞的抑制增殖作用弱于非那雄胺阳性对照组(28.00%vs69.88%),差异有显著性(P<0.05)。流式细胞术分析结果表明,与阴性对照组、空白对照组相比,红三叶总异黄酮浓度达到25.0μg/ml时能够诱导前列腺基质细胞的凋亡,凋亡率为(18.54±2.50)%(P<0.01)。结论:红三叶总异黄酮对前列腺基质细胞有较明显的抑制生长,促进凋亡作用。

Objective：To evaluate the effects of red clover isoflavones on the proliferation and apoptosis of human benign prostatic hyperplasia （BPH） stromal cells. Methods：We treated human prostate stromal cells with red clover isoflavones at the concentration of 12.5,25,50 and 100 μg/ml,and established a PBS blank control,a dimethyl sulphoxide （DMSO） negative control and four finasteride positive control groups （at the concentration of 12.5,25.0,50.0 and 100.0 μg/ml）. We determined the effects of different concentrations of red clover isoflavones on the proliferation of the cells by MTT assay and on their apoptosis by Annexin V/PI double staining flow cytometry. Results：Red clover isoflavones inhibited the proliferation of the BPH stromal cells by 18.86% at 25.0 μg/ml,compared with 5.17% in the blank control group （P 〈0.05）,and more obviously at a higher concentration. At 50.0 μg/ml,red clover isoflavones exhibited a weaker inhibitory effect than finasteride （28% vs 69.88%,P 〈0.05）. Annexin V / PI double staining flow cytometry showed that red clover isoflavones at 25.0 μg/ml induced the apoptosis of the prostate stromal cells by （18.54±2.5）%,with significant differences from the negative control and blank control （P〈0.01）. Conclusion：Red clover isoflavones can inhibit the proliferation and promote the apoptosis of human BPH stromal cells.