米糠油脱臭馏出物中甾醇的分析检测

Determination of sterols in rice bran oil deodorizer distillate

测定了米糠油脱臭馏出物的主要理化指标,并利用分光光度法、HPLC法测定米糠油脱臭馏出物中甾醇含量。结果表明,米糠油脱臭馏出物中饱和脂肪酸含量为23.85%,不饱和脂肪酸含量为74.47%,其中油酸为41.38%,亚油酸为31.74%,亚麻酸为1.35%,与米糠油的脂肪酸组成基本一致;通过分光光度计法测定总甾醇含量为1.41%,通过HPLC测定豆甾醇含量为0.23%,β-谷甾醇为0.25%;GC-MS法检测米糠油脱臭馏出物中还含有3,5-二烯豆甾烷、麦角甾-5烯-3醇,还有一些不经常见到的羊毛甾醇和日耳曼甾醇。

The mainly physical and chemical indexes of the rice bran oil deodorizer distillate were detected, and the content of sterols was analyzed by visual spectrophotometry and HPLC. The results showed that in the deodorizer distillate the saturated fatty acid content was 23.85 % ;and the unsaturated fatty acid content was 74.47% ,including 41.38% oleic acid,31.74% linoleic acid and 1.35% linolenic acid. The fatty acid composition of deodorizer distillate was in accordance with the rice bran oil. The sterols content of the deodorizer distillate was 1.41% by visual spectrophotometry. HPLC analysis showed that the stigmasterol and/3 - sitosterol content was 0.23 % and 0.25 % , respectively. The 3,5 - diene stigmasteran, ergostane -5 - diene -3 -en, lanosterine and germanicol were detected through GC - MS.