微孔草籽油脂肪酸的HPLC-MS分析

HPLC-MS analysis of fatty acids in Microula sikkimensis Hemsl seed oil

采用柱前衍生-高效液相色谱荧光检测法(HPLC-FLD)分析了超临界CO2萃取得到的微孔草籽油的脂肪酸成分。用荧光衍生试剂1-[2-(对甲苯磺酸酯)乙基]-2-苯基咪唑[4,5-f]9,10-菲(TSPP)作为柱前衍生试剂,对39种饱和与不饱和脂肪酸标准品进行衍生,经梯度洗脱实现了39种脂肪酸衍生物完全分离,使用外标法定量,建立了同时测定17种脂肪酸绝对含量的方法。结果表明,微孔草籽油中油酸、亚油酸、亚麻酸相对含量分别是29.09%、23.85%、20.25%;不饱和脂肪酸占总脂肪酸的相对含量为73.19%。

Fatty acids in Microula sikkimensis Hemsl seed oil extracted by supercritical CO2 extraction were analyzed by HPLC fluorescence detection （ HPLC - FLD） with pre - column derivatization. After derivatizing 39 kinds of saturated and unsaturated fatty acids with the pre - column derivatization reagent of 1 - [ 2 - （ p - toluenesulfonate） ethyl ] - 2 - phenylimidazole [ 4,5 - f ] 9,10 - phenanthrene （ TSPP ）, all the fatty acid derivatives were separated with a good baseline resolution in conjunction with a gradient elution. The external standard method for the simultaneous quantitative determination of 17 fatty acids was developed and applied for the determination of the fatty acid contents in Microula sikkimensis Hemsl seed oil samples. The results indicated that the relative content of oleic acid,linoleic acid and linolenic acid in Microula sikkimensis Hemsl seed oil was up to 29.09% ,23.85% and 20.25% ,respectively,and the unsaturated fatty acids of the total fatty acids was 73. 19%.