Bcl基因与Ki-67抗原在胃癌组织中表达的研究

Study on Bcl-2 gene and Ki-67 Expression in Gastric Cancer

目的:探讨bcl-2基因和Ki-67抗原在胃癌中的表达及其可能的作用和意义.方法:用免疫组织化学LSAB法检测bCI-2和Ki-67在胃癌中的表达.结果:正常胃粘膜中bcl-2基本无表达.慢性萎缩性胃粘膜中可见基底部有bcl-2表达.7例胃癌bcl-2表达阳性,其中肠型胃癌阳性高于弥漫型.bcl-2的表达与胃癌的浸润深度和淋巴结转移无关.弥漫型胃癌的Ki-67表达显著高于肠型胃癌.Ki-67指数随浸润深度的增加而增高.有淋巴结转移的Ki-67指数显著高于无转移者.结论:bcl-2的表达可能对胃癌特别是肠型胃癌的发生有重要作用.Ki-67的表达与胃癌的发展及其预后的判断可能有意义.

To investigate the expression of bcl-2 and Ki-67 antigens and their possible role in the genesis and evolution of gastric cancer. Methods: Detection of bcl-2 and Ki-67 expression carried out in gastric cancer patients using immunohistochemical(LSAB) technique. Results: Bcl-2 could be detected in the chronic atrophic gastric mucosa but not in normal mucosa. Bcl-2 expression was higher in intestinal type of gastric cancer(GC) than in diffuse type. No correlation was observed between bcl-2 expression and the depth of invasion as well as lymph node status. Compared with the intestinal type, the diffuse type had a significantly higher Ki-67 index. A trend of increasing Ki-67 index with depth of invasion was noted. The Ki-67 index in GC with lymph node metastasis was significantly higher than those without lymph node metastasis. Conclusions: The present study indicates that aberrant bcl-2 expression may play a role in the genesis of GC. especially in the intestinal type. Ki-67 expression could possibly be a good marker relating to tumor prognosis.