胃癌及其癌旁病变中p16、nm23-H_l基因蛋白的表达及意义

Expression of p16 and nm23-H_1 Protein in Gastric Carcinoma

目的:应用免疫组化方法探讨p16蛋白在胃癌发生中的作用.方法:应用免疫组化方法对57例胃癌切除标本进行p16蛋白和nm23-H_1基因产物的表达检测.结果:57例胃癌中p16蛋白表达阳性率为40/57(70.2%),显著低于癌旁正常胃粘膜(P<0.01).胃癌高分化组的阳性率显著高于低分化组(P<0.05),不同临床分期(UICC分期)及有无淋巴结转移的胃癌病例中,p16蛋白的表达差异无显著性.癌旁胃粘膜肠上皮化生及不典型增生病变中的p16蛋白阳性率介于胃癌及正常胃粘膜之间.肿瘤转移抑制基因nm23-H_1在胃癌的阳性率为41/57(71.9%).不同组织学分级及淋巴结转移的病例中有显著性差异.p16蛋白和nm23-H_1在胃癌的表达呈一定的正相关.结论:本研究认为p16蛋白在胃癌发生中起一定作用.

To investigate the expression of p!6 protein in the carcinogenesis of human gastric carcinoma. Methods: The expression of p16 protein and nm23-H1 gene products were studied immunohistochemically in 57 cases of gastric carcinoma. Results: The positive rates of p16 protein were 70.2%(40/57) in gastric carcinoma tissue and 92.9%(53/57) in the normal surrounding tissue(P< 0.01); the positive rate also showed a significant difference in the well and poorly differentiated group(P<0.05); but no difference was found in group of different clinical stage with or without lymph node metastasis. nm23-H1 protein showed a positive rate of 71.9%(41/57) gastric carcinoma, it was related to the differentiation status, but also related to the lymph node metastasis of gastric carcinoma. p16 and nm23-H1 protein expression showed a positive correlationship. Conclusions: p16 protein may play a role in the carcinogenesis of human gastric carcinoma.