期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

β-synuclein真核表达载体的构建 被引量：1

下载PDF
导出
摘要 目的克隆人β-synuclein基因并构建真核表达载体pcDNA3.1-β-synuclein,为帕金森病发病机制的研究奠定基础。方法从人胎脑组织中提取总RNA,用RT-PCR方法获得人β-synuclein基因的全长cDNA,将其插入pCUm-T载体中;序列测定后,重组携带β-synuclein基因的表达载体pcDNA3.1-β-synuclein。结果经限制性内切酶酶切分析、PCR和DNA序列测定证实目的基因已插入重组质粒。结论成功构建了β-synuclein真核表达载体pcDNA3.1-β-synuclein;该载体可用于帕金森病的相关研究。
作者 郭军堂 苗新东 刘同美 王婷 陈安琪 崔晓栋
机构地区 潍坊医学院
出处 《山东医药》 CAS 北大核心 2010年第15期52-53,共2页 Shandong Medical Journal
基金 山东省卫生厅青年基金项目(2007QW007) 潍坊医学院博士启动基金项目
关键词 β-synuclein基因 帕金森病
分类号 R743 [医药卫生—神经病学与精神病学]
  • 相关文献

参考文献8

  • 1张克忠,蒋雨平.α-synuclein与帕金森病[J].中国临床神经科学,2004,12(4):434-437. 被引量：4
  • 2Goedert M.Alpha-synuclein and neurodegenerative diseases[J].Nat Rev Neurosci,2001,2(7):492-501.
  • 3Yamin G,Munishkina LA,Karymov MA,et al.Forcing nonamyloidogenic beta-synuclein to fibrillate[J].Biochemistry,2005,44(25):9096-9107.
  • 4Fan YX,Pornprot L,Murray VJ,et al.β-synuclein modulates α-synuclein neurotoxicity by reducingα-synuclein protein expression[J].Hum Mol Genet,2006,15(20):3002-3011.
  • 5Windishch M,Hutter-Paier B,Rockenstein E,et al.Development of a New Treatment for Alzheimer's Disease and Parkinson's disease Using Anti-Aggregatory β-synuclein-Derived Peptides[J].J Mol Neurosci,2002,19(1-2):63-69.
  • 6Bertoncini CW,Rasia RM,Lamberto GR,et al.Structural Characterization of the Intrinsically Unfolded Protein β-Synuclein,a Natural Negative Regulator of α-Synuclein Aggregation[J].J Mol Biol,2007,372(3):708-722.
  • 7Lee DY,Paik SR,Choi KY.β-Synuclein exhibits chaperone activity more efficiently than а-synuclein[J].FEB Slett,2004,576(1-2):256-260.
  • 8Hashimoto M,Rockenstein E,Mante M,et al.An antiaggregation gene therapy strategy for lewy body disease utilizing β-synuclein lentivirus in a transgenic model[J].Gene Ther,2004,11(23):1713-1723.

二级参考文献18

  • 1Ploymeropoulos MH,Lavedan C,Leroy E,et al.Mutation in the alpha-synuclein gene identified in families with Parkinson's disease[J].Science,1997,276:2405-2407
  • 2Trojanowski JQ,Lee VM."Fretal attractions"of proterins.A comprehensive hypothetical mechanism underlying Alzheimers's disease and other neurodegenerative disorders[J].Ann NY Acad Sci,2000,924:62-67
  • 3Vladimir N,Lie J,Anthony L,et al.Metal-triggered structural transformation aggregation and fibrillation of human alpha-synuclein[J].J Biol Chem,2001,276:44284-44294
  • 4Uversky VN,Li J,Fink AL.Pesticides directly accelerate the tate of alpha-synuclein fibril formation:a possible factor in Parkinson's disease[J].FEBS Lett,2001,500:105-108
  • 5Bennett MC,Bishop JF,Leng Y,et al.Degradation of α-synuclein by proteasome.J Biol Chem[J].1999,274:33855-33858
  • 6McNaught KS,Jenner P.Proteasomal function is impaired in substantia nigra in Parkinson′s disease[J].Neurosici Lett,2001,297:191-194
  • 7McNaught KS,Mytilineou C,Baptiste RJ,et al.Impairment of the ubiquitin-proteasome causes dopaminergic cell death and inclusion body formation in ventral mesencephlic cultures[J].J Neuro Chem,2002,81:301-306
  • 8Bence NF,Sampat RM,Kopito RR,et al.Impairment of the ubiquitin-proteasome system by protein aggregation[J].Science ,2001,292;1552-1555
  • 9Uversky VN,Li J,Anthony L,et al.Biophysical properties of the synucleins and their propensities to fibrillate:inhibition of alpha-synuclein assembly by β- and γ-synuceins[J].J Biol Chem,2002,277:11970-11978
  • 10Feany MB,Bender WW.A drosophila model of Parkinson's disease[J].Nature,2000,404;394-398

共引文献3

同被引文献13

  • 1Tobe T, Nakajo S, Tanaka A, et al. Cloning and characterization of the eDNA encoding a novel brain-specific 14- kDa protein. J Neurochem,1992;59(5) :1624-1629.
  • 2Nakajo S, Tsukada K, Omata K, et al. A new brain-specific 14-kDa protein is a phosphoprotein. Its complete amino acid sequence and evidence for phosphorylation. Eur J Bioehem, 1993,217(3):1057 -1063.
  • 3Iwai A, Masliah E, Yoshimoto M, et al. The precursor protein of non a beta component of Alzheimer's disease amyloid is a presynaptic protein of the central nervous system. Neuron, 1995,14(2) :467-475.
  • 4Nakajo S, Shioda S, Nakai Y, et al. Localization of phosphoneuroprotein 14 (PNP 14) and its mRNA expression in rat brain determined by immunocytochemistry and in situ hybridization. Brain Res Mol Brain Res,1994,27(1):81-86.
  • 5Lavedan C. The synuclein family. Genome Res, 1998, 8 (9) 871-880.
  • 6George J. The synueleins. Genome Biol,2002,3(7):1-6.
  • 7Nakajo S, Omata K, Aiuchi T, et al. Purification and characterization of a novel brain-specific 14-kDa protein. J Neurochem, 990 , 55 (6) : 2031-2038.
  • 8Yoshida H, Craxton M, Jakes R, et al. Synuelein proteins of the pufferfish Fugu rubripes: sequences and functional characterization. Biochemistry, 2006 ; 45 (8) : 2599-2607.
  • 9西弗HL,格兰杰RM,哈兰RM.爪蟾早期发育实验指南.北京:科学出版社,2004:249274.
  • 10Martin G, Goh E, Neff AW. Evaluation of the developmental toxicity of crocetin on Xenopus. Food Chem Toxicol, 2002;40 (7) :959-964.

引证文献1

山东医药
2010年 第15期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部