葡萄糖对NIT-1细胞激素敏感性脂肪酶的影响

Effect of glucose on hormone sensitive lipase in NIT-1 cell

以不同浓度葡萄糖孵育NIT-1细胞不同时间，结果显示高糖环境下细胞内甘油三酯含量呈浓度和时间依赖性增加（P〈0．05），高糖对激素敏感性脂肪酶（HSL）mRNA和蛋白表达的影响呈现先增加后降低的抛物线型作用，脂肪分解呈相似变化。提示HSL对葡萄糖浓度的适应性变化可能在调节胰岛细胞内脂质含量和糖脂毒性中起重要作用。

NIT-1 cells were exposed to various concentrations of glucose for 24, 48, and 72 hours. The content of triglyceride in NIT-1 cells increased in dbse- and time-dependent manners （P〈 0. 05 ）. Long-term exposure of NIT-1 ceils to high glucose concentrations caused an inverse ＂v＂-like induction of HSL mRNA and protein expressions, which increased from beginning, and then decreased, along with similar changes of lipolysis. These results suggest that the adaptation of HSL may play an important role in regulating the intracellular triglyceride pool and the development of glucolipotoxicity in pancreatic β ceils.