应用基因芯片技术探讨乌灵菌粉的抗抑郁机制

Anti-depressant Mechanisms of Wuling Jun Powder by Gene Microarray Analysis

目的采用基因芯片技术探讨乌灵菌粉的抗抑郁作用机制。方法以慢性轻度应激(CMS)致抑郁模型小鼠为观察对象,提取对照组、CMS组、乌灵菌粉组、氟西汀治疗组大脑皮层的总RNA,应用Clontech公司的小鼠1.2表达谱芯片进行各组间1176个基因的比较。结果基因芯片比较发现,CMS组小鼠与对照组相比皮层有130个基因发生改变,其中上调116个,下调14个;与CMS组比较,乌灵菌粉组有85个基因发生改变,其中上调34个,下调51个;氟西汀组133个基因发生改变,上调35个,下调98个。这些基因涉及受体活性、蛋白激酶、炎性因子、转铁蛋白、神经发生等各个方面。结论CMS致抑郁模型小鼠脑内多种功能基因的表达出现亢奋或低下,而通过乌灵菌粉和氟西汀干预后可使多个基因表达趋向正常水平。

Objective To explore the possible mechanisms of Wuling Jun Power by the DNA microarray technique.Methods An experimental depression model was established by exposing the mouse to a chronic mild stress procedure. The total RNA was extracted reverse-transcripted and hybrided to the mouse 1.2 cDNA microarray （Clontech）. The difference of expression profiles between control model,Wuling Jun powder and fluoxetine-treated groups were analyzed by the Image 2.1 Software.Results 130 genes were significantly altered in stress group compared with the control groups. Among them,116 genes were up-regulated and 14 genes were down-regulated. Meanwhile,85 genes significantly changed in the Wuling Jun powder treated group with 34 genes up-regulated and 51 genes down-regulated compared with the model groups. For the Fluoxetine-treated group,133 genes significantly changed with 35 genes up-regulated and 98 genes down-regulated compared with the model groups. These genes were associated with many aspects of life including receptor activity,protein kinases,inflammatory factors,transferrin,neurogenesis and so on.Conclusion Multiple genes were affected by the stress exposure. Altered changes of some genes were normalized by Wuling Jun powder and Fluoxetine treatment. In general,the mechanisms of Wuling Jun powder and Fluoxetine are similar,but also with minor difference.