创伤后应激障碍模型大鼠内侧前额皮质磷酸化细胞外信号调节激酶1/2及c-fos的变化

Changes of phosphorylated ERK 1/2 and c-fos in the medial prefrontal cortex of post-traumatic stress disorder rats

目的探讨创伤后应激障碍(PTSD)模型大鼠内侧前额皮质(mPFC)磷酸化细胞外信号调节激酶1/2(pERK1/2)和c-fos的表达变化。方法将30只成年健康雄性Wistar大鼠随机分为对照组(15只)和PTSD模型组(15只),采用无连续单一应激(SPS)方法制备PTSD模型。用免疫组织化学和免疫印迹法检测mPFCpERK1/2的表达变化,RT-PCR检测c-fosmRNA表达变化。结果免疫组织化学分析显示,对照组和模型组pERK1/2阳性细胞数分别为10.4±2.07、48.8±10.08,阳性信号吸光度值分别为24.955±3.691、110.810±10.643,差异有统计学意义(P<0.01);免疫印迹分析显示,对照组和模型组pERK/2相对表达量分别为0.510±0.052和1.109±0.106,差异有统计学意义(P<0.01);RT-PCR分析结果显示,对照组和模型组c-fosmRNA相对表达分别为0.267±0.067和1.049±0.131,差异有统计学意义(P<0.01)。结论mPFCpERK1/2和c-fos表达增高,可能参与了PTSD模型大鼠的病理生理过程。

Objective To observe the changes of phosphorylated extracellular signalregulated kinase 1/2 （pERK1/2） and c-fos in the medial prefrontal cortex （mPFC） of post-traumatic stress disorder （PTSD） rats. Methods Male Wistar rats were randomly divided into control group and PTSD model group. The model group rats were exposed to the singleprolonged stress （SPS） to set up the rat PTSD model. The expression of pERK1/2 was detected using immunohistochemistry and Western blotting, and the expression of c-fos mRNA was detected using reverse transcription polymerase chain reaction （RT-PCR）. Results The result of immunohistochemistry analysis showed that the number of pERK1/2-positive cells of control group and model group were 10.4±2.07 and 48.8±10.08 respectively （P〈0.01）, and integral optical density were 24.955±3.691 and 110.810±10.643 respectively （P〈0.01）. And Western blotting showed that relative expression quantities of pERK/β actin were 0.510±0.052 and 1.109±0.106 respectively （P〈0.01）. The quantities of c-fos mRNA relative expression of control group and model group were 0.267±0.067 and 1.049±0.131 （P〈0.01）. Conlusion The levels of pERK1/2 and c-fos increase significantly in mPFC of PTSD model rats. The ERK signal transduction pathway in mPFC might play an important role in the pathogenesis of PTSD.