促甲状腺激素释放激素受体1和2在二甲磺酸乙烷处理大鼠睾丸中的表达及意义

Expression and significance of TRH-R1 and TRH-R2 in the testis of EDS-treated adult rats

目的探讨促甲状腺激素释放激素受体-1,-2(TRH-R1,TRH-R2)在二甲磺酸乙烷(EDS)处理大鼠睾丸中的表达及其在Leydig细胞中表达的意义。方法构建30只EDS处理大鼠模型,应用蛋白质免疫印迹杂交技术、免疫组织化学ABC法以及免疫荧光双标法,检测TRH-R1和TRH-R2在EDS处理后2d、7d、14d、21d和28d大鼠睾丸中的表达和定位。结果免疫印迹杂交发现,EDS处理后2d至14d,大鼠睾丸组织未见TRH-R1和TRH-R2表达,在EDS处理后21d、28d再次检测到了TRH-R1和TRH-R2的表达;免疫组织化学染色显示,TRH-R1和TRH-R2表达于EDS处理后21d、28d大鼠睾丸生精小管周围的长梭形细胞,免疫荧光双标证实此类细胞为再生的Leydig祖细胞。结论TRH-R1和TRH-R2参与了Leydig细胞的再生过程,可能对成年型Leydig细胞的发育、分化具有调节作用。

Objective To investigate the expression of thyrotropinreleasing hormone receptor （TRH-R） type-1 and type-2 in ethane dimethanesulphonate （EDS）treated rat testis, and to discuss the significance of its expression in Leydig cells. Methods To make the injured testis Leydig cells rat model with EDS treatment. Western blotting, immunohistochemical ABC and immunofluorescence double labeling methods were used to detect the expression and location of TRH-R1 and R2 in the testicular tissues of EDStreatedday 2,day 7,day 14,day 21 and day 28 rat mode, respectively. Results Western blotting results showed that the positive immunochemical staining was not found in the testicular tissues of the EDS-treated day 2 to day 14, on the other hand,they were found in EDS-treated-21 day and EDS-treated-28 day. Immunohistochemistry demonstrated that TRH-R1 and R2 expressed in the spindleshaped cells reappeared around seminiferous tubules of postEDS 21 days and 28 days groups. Immunofluorescence double labeling confirmed that these TRH-R1 and R2 positively stained cells were newly regenerated progenitor Leydig cells. Conclusion TRH-R1 and R2 are involved in the regeneration of Leydig cells in EDStreated rat testis, and they may exert functions in the proliferation and differentiation of adult type Leydig cells.