大鼠肝再生中8种肝脏细胞的细胞免疫相关基因转录谱预示的生理活动

Transcript atlas of cell immunity-associated gene uncovers physiological activities in 8 liver cell types of rat regenerating liver

目的了解大鼠肝再生中8种肝脏细胞的细胞免疫相关基因转录谱,及其预示的细胞免疫活动。方法用Percoll密度梯度离心结合免疫磁珠分选方法分离大鼠再生肝的肝细胞、胆管上皮细胞、卵圆细胞、星形细胞、窦内皮细胞、库普弗细胞、陷窝细胞和树突状细胞等8种细胞,用RatGenome2302.0芯片等检测细胞免疫相关基因在上述细胞中表达变化,用Cluster等软件及生物信息学和系统学生物等方法分析它们的表达模式及预示的生理活动。结果大鼠肝再生中40个细胞免疫相关基因发生了表达变化,相应细胞的基因数为19、19、9、19、19、21、22、21。肝再生启动阶段和进展阶段抗原肽-MHC复合物形成,NF-κB激酶活性和IL-2等细胞因子合成增加,终止阶段NF-κB促进细胞分化活动和caspase诱导T细胞凋亡活动增强。结论大鼠肝再生与细胞免疫相关。

Objective To study the transcript atlas of cell immunityassociated genes in 8 liver cell types including hepatocytes, bile duct epithelial cells, oval cells, hepatic stellate cells, sinus endothelial cells, Kupffer cells, pit cells and dendritic cells from the rat regenerating liver. Methods The 8 liver cell types were isolated respectively by Percoll density gradient centrifugation combined with immune magnetic beads separation method. Their expression changes in the regenerating livers were detected by Rat Genome 230 20 Array, the expression patterns and the predicted physiological activities of cell immunityassociated genes were analyzed by Cluster program, and the methods of bioinformation and systematic biology. Results A total of 40 cell immunityassociated genes yielded the meaningful expression changes in liver regeneration, the corresponding gene numbers in 8 liver cell types were 19, 19, 9, 19, 19, 21, 22 and 21, respectively. It suggested that the formation of antigen peptide-MHC complexes, the NF-κB kinase activity and the production of cytokines like IL-2 were enhanced at the priming and progressing phases of liver regeneration. The biological activities, such as NF-κBpromoting cell differentiation and Caspase-induced T cell apoptosis, were elevated at termination phase. Conclusion The rat liver regeneration is associated with cell immunity.