大鼠肝再生中肝脏细胞的基因转录谱预示AI408225促进抗原肽与TCR结合

Gene transcript profiles of liver cells predict that AI408225 promotes antigen peptide combining with TCR in rat regenerating liver

目的了解新基因AI408225与大鼠肝再生涉及的体液免疫相关性。方法大鼠再生肝8种细胞的分离,采用Percoll密度梯度离心结合免疫磁珠分选方法进行,用MicrosoftExcel、BLAST等软件分析基因的序列同源性及共表达关系,用生物信息学和系统生物学等方法分析新基因参与的生理活动。结果93个参与体液免疫的已知基因与肝再生相关,肝细胞、胆管上皮细胞、卵圆细胞、肝星形细胞、窦内皮细胞、库普弗细胞、陷窝细胞、树突状细胞的体液免疫相关基因数为33、46、17、59、48、35、53、68。其中,cd4与AI408225同源和共表达。结论大鼠肝再生与体液免疫相关,AI408225参与MHCII-抗原肽-CD4-TCR复合物形成。

Objective To explore the correlation of unknown gene AI408225 and humoral immunity involved in rat regenerating liver. Methods Isolation of rat 8 liver cell types, detection of their genes expression change were accorded to Percoll density gradient centrifugation combined with immune magnetic beads separation method, and their genes sequence homoeology and coexpression relation were analyzed by Microsoft Excel, BLAST software, and the prediction physiological activities were analyzed by bioinformatics and systematic biology. Results A total of 93 humoral immunityass ociated genes yielded the meaningful expression changes in liver regeneration, the corresponding gene numbers in 8 liver cell types included hepatocytes, bile duct epithelial cells, oval cells, hepatic stellate cells, sinus endothelial cells, Kupffer cells, pit cells and dendritic cells were 33, 46, 17, 59, 48, 35, 53, 68 Among thatAI408225 and cd4 are homology and coexpression. Conclusion The rat liver regeneration is closely associated with humoral immunity, and AI408225 participates in the formation of compound of MHC II-antigen peptide-CD4-TCR.