细胞角蛋白和波形蛋白在皮肤淀粉样变淀粉样蛋白中表达的研究

Cytokeratins and vimentin expressions in the amyloids of lichen amyloidosis and macular amyloidosis

目的:探讨皮肤淀粉样变淀粉样蛋白的生物学来源。方法:应用免疫组化染色技术检测4种角蛋白(cytokeratins,CKs)及波形蛋白在20例斑状或苔藓样型皮肤淀粉样变淀粉样蛋白中的表达情况。结果:①CK34βE12和CK5/6在20例标本的淀粉样蛋白团块中均呈阳性表达;而AE1/AE3、CK10/13和波形蛋白在淀粉样蛋白团块中均呈阴性表达;②淀粉样蛋白团块中波形蛋白表达阳性的成纤维细胞明显增生。结论:淀粉样蛋白主要来源于凋亡的表皮基底细胞而非真皮组织。

Objective： To discuss the biological origin of the amyloids in macular amyloidosis （MA） and lichen amyloidosis （LA）. Methods： The immunohistochemical staining was used to investigate the expressions of cytokeratins （CKs） and vimentin in amyloid deposits of formalin-fixed and paraffin-embedded tissue specimens from 13 patients with LA and 7 with MA. Results： In amyloid deposits, only CK34βE12 and CK5/6 was positive in 20 biopsy specimens, and AE1/AE3, CK10/13 and vimentin were negative. Fibroblast cells which could express vimentin presented significant proliferation among the amyloids. Conclusion： The results confirm the hypothesis that the amyloid in lichen and macular amyloidosis mainly derived from basal keratinoeytes, not from dermal tissues.