奥沙利铂处理HepG2细胞后DR5、caspase-8及survivin、bcl-2表达的变化

The expression of DR5,caspase-8,survivin and bcl-2 in HepG2 cell line treated with oxliplatin

目的:研究奥沙利铂作用于HepG2细胞后DR5、caspase-8、survivin、bcl-2的表达,对奥沙利铂诱导HepG2细胞凋亡的机制进行初步探讨。方法:实验分为对照组和奥沙利铂组,应用MTT法、流式细胞仪体外研究奥沙利铂对HepG2细胞增殖的影响;采用免疫细胞化学及western blot的方法,观察奥沙利铂作用于HepG2细胞24h后DR5、caspase-8、survivin、bcl-2的表达情况。结果:奥沙利铂对肝癌HepG2细胞的抑制作用呈时间依赖性和浓度依赖性;奥沙利铂作用于HepG2细胞24小时后细胞表面DR5及caspase-8的表达增强,而survivin和bcl-2的表达减弱。结论:奥沙利铂诱导肝癌HepG2细胞凋亡的机制可能与其上调DR5及caspase-8表达和下调survivin及bcl-2表达有关。

Objective：To research the apoptosis and mechanism induced by oxaliplatin in HepG2 cell line.Methods： HepG2 was treated by virous cencetration of oxotiplatin,cell growth inhibition was assessed by MTT and apoptosis was observed by FMC;the expression of DR5,caspase-8,survivin,bcl-2 was detected by immunohistochemical method and western blot.Results： Oxaliplatin can apparently inhibite the growth of HepG2 and induce apoptosis;when the cell was treated by oxaliplatin for 24h,the expression of DR5 and caspase-8 was up-regulated,the expression of surviving and bcl-2 was down-regulated.Conclution： The cell growth inhibition of oxaliplatin on HepG2 is dose-dependent and time-dependent,the apoptosis was related to up-related of DR5 and caspase-8,as well as the down-regulated of survivin and bcl-2.