APPSWE转基因小鼠发育过程中海马CA1区神经细胞凋亡研究

Study of Neuroapoptosis in Developing Hippocampus of APPSWE Transgenic Mouse

目的探讨APPSWE转基因小鼠发育过程中海马CA1区神经细胞凋亡规律。方法取不同发育时间（P0、P7、P14、P30、P00、P180）APPSWE转基因模型鼠与同时问点对照鼠，Nissl染色观察海马结构和锥体细胞形态，免疫组织化学方法观察海马细胞内Caspase-3表达变化，RT—PCR检测Caspase-3 mRNA表达变化。结果随着小鼠的生长发育，P14时间点以后，模型组CA1区神经元Caspase-3阳性细胞密度比对照组高，RT—PCR检测结果与Caspase-3免疫组织化学结果基本一致。结论APPSWE转基因小鼠发育中的海马神经细胞过度凋亡可能与阿茨海默病的发生、发展具有联系。

Objective To explore the rule of neuroapoptosis in developing hippocampus of APPSWE transgenic mice of Alzheimer＇s disease （AD） . Methods With comparison to wild type mice, Nissl staining, Caspase-3 immunohistochemistry and RT-PCR were used to study neuroapoptosis induction in hippocampus CAI area of APPSWE transgenic mice at various developing time points from postnatal day 0 （PO）, day7 （P7）, day l4 （P14）, day30 （P30）, day90 （P90） to day 180 （P180） . Results At P14, neuroapoptosis in CA1 area of AD model was obviously higher than age-matched controls, as shown consistently by both Caspase-3 immunohistochemistry staining and RT-PCR of Caspase-3 mRNA. Conclusion It was implied that neuroapoptosis in hippocampus ares of APPSWE transgenie mice is most likely related to development of Alzheimer＇ s disease.